Evidence map›Paper›PMID 37241647›Full record

ArticleMicromachines2023

Stopped Flow of Glycerol Induces the Enhancement of Adsorption and Aggregation of HRP on Mica.

Yuri D Ivanov, Ivan D Shumov, Andrey F Kozlov, Maria O Ershova, Anastasia A Valueva, Irina A Ivanova, Vadim Y Tatur, Andrei A Lukyanitsa, Nina D Ivanova, Vadim S Ziborov

Abstract read
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In one paragraph

Article in Micromachines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yuri D IvanovInstitute of Biomedical Chemistry, Pogodinskaya Street, 10 Build. 8, Moscow 119121, Russia.ORCID 0000-0001-5041-1914
Ivan D ShumovInstitute of Biomedical Chemistry, Pogodinskaya Street, 10 Build. 8, Moscow 119121, Russia.ORCID 0000-0002-9795-7065
Andrey F KozlovInstitute of Biomedical Chemistry, Pogodinskaya Street, 10 Build. 8, Moscow 119121, Russia.
Maria O ErshovaInstitute of Biomedical Chemistry, Pogodinskaya Street, 10 Build. 8, Moscow 119121, Russia.
Anastasia A ValuevaInstitute of Biomedical Chemistry, Pogodinskaya Street, 10 Build. 8, Moscow 119121, Russia.
Irina A IvanovaInstitute of Biomedical Chemistry, Pogodinskaya Street, 10 Build. 8, Moscow 119121, Russia.
Vadim Y TaturFoundation of Perspective Technologies and Novations, Moscow 115682, Russia.
Andrei A LukyanitsaFoundation of Perspective Technologies and Novations, Moscow 115682, Russia.
Nina D IvanovaFoundation of Perspective Technologies and Novations, Moscow 115682, Russia.
Vadim S ZiborovInstitute of Biomedical Chemistry, Pogodinskaya Street, 10 Build. 8, Moscow 119121, Russia.

Funding

The Ministry of Science and Higher Education of the Russian Federation within the framework of state support for the creation and development of World-Class Research Centers 'Digital Biodesign and Personalized Healthcare' 075-15-2022-305
6 · The paper itself

Abstract

Glycerol is a usable component of heat-transfer fluids, and is thus suitable for the use in microchannel-based heat exchangers in biosensors and microelectronic devices. The flow of a fluid can lead to the generation of electromagnetic fields, which can affect enzymes. Herein, by means of atomic force microscopy (AFM) and spectrophotometry, a long-term effect of stopped flow of glycerol through a coiled heat exchanger on horseradish peroxidase (HRP) has been revealed. Samples of buffered HRP solution were incubated near either the inlet or the outlet sections of the heat exchanger after stopping the flow. It has been found that both the enzyme aggregation state and the number of mica-adsorbed HRP particles increase after such an incubation for 40 min. Moreover, the enzymatic activity of the enzyme incubated near the inlet section has been found to increase in comparison with that of the control sample, while the activity of the enzyme incubated near the outlet section remained unaffected. Our results can find application in the development of biosensors and bioreactors, in which flow-based heat exchangers are employed.

Indexed as

atomic force microscopyenzyme adsorptionglycerol flowhorseradish peroxidase

Identifiers

PMID37241647
PMCPMC10221182

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.