Evidence map›Paper›PMID 37240176›Full record

ArticleInternational journal of molecular sciences2023

Derivation of Human Corneal Keratocytes from ReLEx SMILE Lenticules for Cell Therapy and Tissue Engineering.

Maria A Surovtseva, Irina I Kim, Natalia A Bondarenko, Alexander P Lykov, Kristina Yu Krasner, Elena V Chepeleva, Nataliya P Bgatova, Alexander N Trunov, Valery V Chernykh, Olga V Poveshchenko

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Maria A SurovtsevaResearch Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0002-4752-988X
Irina I KimResearch Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.
Natalia A BondarenkoResearch Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.
Alexander P LykovResearch Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0003-4897-8676
Kristina Yu KrasnerResearch Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.
Elena V ChepelevaResearch Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0003-0715-4451
Nataliya P BgatovaResearch Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.ORCID 0000-0002-4507-093X
Alexander N TrunovNovosibirsk Branch of S. Fedorov Eye Microsurgery Federal State Institution, 10 Kalkhidskaya Str., 630096 Novosibirsk, Russia.
Valery V ChernykhNovosibirsk Branch of S. Fedorov Eye Microsurgery Federal State Institution, 10 Kalkhidskaya Str., 630096 Novosibirsk, Russia.
Olga V PoveshchenkoResearch Institute of Clinical and Experimental Lymphology-Branch of the Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, 2 Timakova Str., 630060 Novosibirsk, Russia.
Russian Academy of Sciences · RUIntersectoral Research and Technology Complex Eye Microsurgery · RU

Funding

CSRD VA 1
6 · The paper itself

Abstract

Fibroblasts isolated and expanded from ReLEx SMILE lenticules can be a source of human keratocytes. Since corneal keratocytes are quiescent cells, it is difficult to expand them in vitro in suitable numbers for clinical and experimental use. In the present study, this problem was solved by isolating and growing corneal fibroblasts (CFs) with a high proliferative potential and their reversion to keratocytes in a selective serum-free medium. Fibroblasts reversed into keratocytes (rCFs) had a dendritic morphology and ultrastructural signs of activation of protein synthesis and metabolism. The cultivation of CFs in a medium with 10% FCS and their reversion into keratocytes was not accompanied by the induction of myofibroblasts. After reversion, the cells spontaneously formed spheroids and expressed keratocan and lumican markers, but not mesenchymal ones. The rCFs had low proliferative and migratory activity, and their conditioned medium contained a low level of VEGF. CF reversion was not accompanied by a change with the levels of IGF-1, TNF-alpha, SDF-1a, and sICAM-1. In the present study, it has been demonstrated that fibroblasts from ReLEx SMILE lenticules reverse into keratocytes in serum-free KGM, maintaining the morphology and functional properties of primary keratocytes. These keratocytes have a potential for tissue engineering and cell therapy of various corneal pathologies.

Indexed as

Corneal KeratocytesTissue EngineeringCell- and Tissue-Based TherapyCells, CulturedCorneal StromaFibroblastsHumanscorneal fibroblastscytokineskeratocytesmigrationproliferationreversion

Identifiers

PMID37240176
PMCPMC10218243
OpenAlexW4377041985

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.