Evidence map›Paper›PMID 37237672›Full record

ArticleBioengineering (Basel, Switzerland)2023

Scalable and High-Throughput In Vitro Vibratory Platform for Vocal Fold Tissue Engineering Applications.

Andreea Biehl, Ramair Colmon, Anastasia Timofeeva, Ana Maria Gracioso Martins, Gregory R Dion, Kara Peters, Donald O Freytes

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioengineering (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Andreea BiehlJoint Department of Biomedical Engineering, North Carolina State University & University of North Carolina-Chapel Hill, 4130 Engineering Building III, Campus Box 7115, Raleigh, NC 27695, USA.ORCID 0000-0002-0881-1014
Ramair ColmonJoint Department of Biomedical Engineering, North Carolina State University & University of North Carolina-Chapel Hill, 4130 Engineering Building III, Campus Box 7115, Raleigh, NC 27695, USA.
Anastasia TimofeevaDepartment of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USA.ORCID 0000-0003-4184-6857
Ana Maria Gracioso MartinsJoint Department of Biomedical Engineering, North Carolina State University & University of North Carolina-Chapel Hill, 4130 Engineering Building III, Campus Box 7115, Raleigh, NC 27695, USA.
Gregory R DionDepartment of Otolaryngology-Head and Neck Surgery, University of Cincinnati, Cincinnati, OH 45267, USA.
Kara PetersDepartment of Mechanical and Aerospace Engineering, North Carolina State University, Raleigh, NC 27695, USA.ORCID 0000-0002-1945-7196
Donald O FreytesJoint Department of Biomedical Engineering, North Carolina State University & University of North Carolina-Chapel Hill, 4130 Engineering Building III, Campus Box 7115, Raleigh, NC 27695, USA.ORCID 0000-0003-4636-5809

Funding

Optimal Tissue Engineering Approach for Treating Injured Vocal FoldsR01DC017139 · NIDCD · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI FREYTES, DONALD O · 2019 to 2023
$2.7M
Augmenting Vocal Fold Repair Using an Optimized Lamina Propria Extracellular Matrix Derived HydrogelR01DC017743 · NIDCD · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI FREYTES, DONALD O · 2019 to 2023
$2.7M
Training Grant in Comparative Molecular MedicineT32GM133393 · NIGMS · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI Matthew B Fisher, Caroline Laplante · 2021 to 2026
$1.7M
NIDCD NIH HHS R01 DC017139NIDCD NIH HHS R01DC017139, R01DC017743NIDCD NIH HHS R01 DC017743NIGMS NIH HHS T32 GM133393
6 · The paper itself

Abstract

The vocal folds (VFs) are constantly exposed to mechanical stimulation leading to changes in biomechanical properties, structure, and composition. The development of long-term strategies for VF treatment depends on the characterization of related cells, biomaterials, or engineered tissues in a controlled mechanical environment. Our aim was to design, develop, and characterize a scalable and high-throughput platform that mimics the mechanical microenvironment of the VFs in vitro. The platform consists of a 24-well plate fitted with a flexible membrane atop a waveguide equipped with piezoelectric speakers which allows for cells to be exposed to various phonatory stimuli. The displacements of the flexible membrane were characterized via Laser Doppler Vibrometry (LDV). Human VF fibroblasts and mesenchymal stem cells were seeded, exposed to various vibratory regimes, and the expression of pro-fibrotic and pro-inflammatory genes was analyzed. Compared to current bioreactor designs, the platform developed in this study can incorporate commercial assay formats ranging from 6- to 96-well plates which represents a significant improvement in scalability. This platform is modular and allows for tunable frequency regimes.

Indexed as

bioreactordisplacementfibroblastsfrequencygene expressionmesenchymal stem cellspiezoelectric speakervibrationvocal fold

Identifiers

PMID37237672
PMCPMC10215097

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.