Evidence map›Paper›PMID 37237379›Full record

ArticleFluids and barriers of the CNS2023

Mouse embryonic stem cell-derived blood-brain barrier model: applicability to studying antibody triggered receptor mediated transcytosis.

Anna Jezierski, Jez Huang, Arsalan S Haqqani, Julie Haukenfrers, Ziying Liu, Ewa Baumann, Caroline Sodja, Claudie Charlebois, Christie E Delaney, Alexandra T Star and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Fluids and barriers of the CNS, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.4field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 1 institution in 1 country.

Anna Jezierski *Human Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada. anna.jezierski@nrc-cnrc.gc.ca.
Jez Huang *Human Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Arsalan S HaqqaniHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Julie HaukenfrersHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Ziying LiuHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Ewa BaumannHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Caroline SodjaHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Claudie CharleboisHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Christie E DelaneyHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Alexandra T StarHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Qing LiuHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
Danica B StanimirovicHuman Health Therapeutics Research Centre, National Research Council of Canada, ON, Ottawa, Canada.
National Research Council Canada · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Blood brain barrier (BBB) models in vitro are an important tool to aid in the pre-clinical evaluation and selection of BBB-crossing therapeutics. Stem cell derived BBB models have recently demonstrated a substantial advantage over primary and immortalized brain endothelial cells (BECs) for BBB modeling. Coupled with recent discoveries highlighting significant species differences in the expression and function of key BBB transporters, the field is in need of robust, species-specific BBB models for improved translational predictability. We have developed a mouse BBB model, composed of mouse embryonic stem cell (mESC-D3)-derived brain endothelial-like cells (mBECs), employing a directed monolayer differentiation strategy. Although the mBECs showed a mixed endothelial-epithelial phenotype, they exhibited high transendothelial electrical resistance, inducible by retinoic acid treatment up to 400 Ω cm

Indexed as

Blood-Brain BarrierInduced Pluripotent Stem CellsAnimalsAntibodiesEndothelial CellsHumansMiceMouse Embryonic Stem CellsTranscytosisAntibodiesAntibodiesApparent permeabilityBlood brain barrierEmbryonic stem cellsMouse brain endothelial cellsReceptor mediated transcytosis

Identifiers

PMID37237379
PMCPMC10224255
OpenAlexW4378470736

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.