Evidence map›Paper›PMID 37235413›Full record

ArticleVeterinary sciences2023

Development of a Crystal Digital RT-PCR for the Detection of Atypical Porcine Pestivirus.

Huixin Liu, Kaichuang Shi, Shuping Feng, Yanwen Yin, Feng Long, Hongbin Si

Open access · goldAbstract read
In one paragraph

Article in Veterinary sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 2 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Huixin LiuCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.
Kaichuang ShiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.ORCID 0000-0002-1023-9635
Shuping FengGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Yanwen YinGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.
Feng LongGuangxi Center for Animal Disease Control and Prevention, Nanning 530001, China.ORCID 0000-0002-9625-0527
Hongbin SiCollege of Animal Science and Technology, Guangxi University, Nanning 530005, China.ORCID 0000-0002-0416-6150
Guangxi Center for Disease Prevention and Control · CNGuangxi University · CN

Funding

Guangxi Science and Technology Bureau, China No. AB21238003
6 · The paper itself

Abstract

Atypical porcine pestivirus (APPV), a newly discovered virus, is associated with the type A-II congenital tremor (CT) in neonatal piglets. APPV distributes throughout the world and causes certain economic losses to the swine industry. The specific primers and probe were designed targeting the 5' untranslated region (UTR) of APPV to amplify a 90 bp fragment, and the recombinant standard plasmid was constructed. After optimizing the concentrations of primers and probe, annealing temperature, and reaction cycles, a crystal digital RT-PCR (cdRT-PCR) and real-time quantitative RT-PCR (qRT-PCR) were successfully established. The results showed that the standard curves of the qRT-PCR and the cdRT-PCR had R

Indexed as

atypical porcine pestivirus (APPV)crystal digital RT-PCR (cdRT-PCR)detection methodreal-time quantitative RT-PCR (qRT-PCR)

Identifiers

PMID37235413
PMCPMC10223196
OpenAlexW4378516099

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.