ArticleFrontiers in neuroanatomy2023
A novel immunohistochemical protocol for paraffin embedded tissue sections using free-floating techniques.
Article in Frontiers in neuroanatomy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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5 citing papers in PubMed.
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- Terminalia arjuna Bark Nanosuspension Attenuates Fluoride-Induced Cardiotoxicity in Rats by Modulating Oxidative Stress, Inflammation and ACE Expression.Biological trace element research · 2026Article
- A Water Extract of Mixed Mushroom Mycelia Mitigates Cognitive Deficit and Oxidative Stress After Global Cerebral Ischemia-Reperfusion Injury.Current issues in molecular biology · 2026Article
- Assessing the diagnostic potential of SATB2 and β-catenin as biomarkers and therapeutic targets in pancreatic ductal adenocarcinoma.Journal of cancer research and clinical oncology · 2025Article
- Concomitant use of pre-emptive analgesia with local and general anesthesia in rat uterine pain surgical model.Molecular painArticle
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Abstract
Immunohistochemistry (IHC) is a well-established and widely used protocol used to visualize tissue architecture, protein expression and localization. Free-floating methods for IHC employ tissue sections that are cut from a cryostat or vibratome. The limitations of these tissue sections are tissue fragility, poor morphology, and the need to use sections of 20-50 μm. In addition, there is a void of information regarding the use of free floating immunohistochemical techniques on paraffin embedded tissue. To address this, we developed a free-float IHC protocol with paraffin embedded tissue (PFFP) that saves time, resources, and tissues. PFFP localized GFAP, olfactory marker protein, tyrosine hydroxylase, and Nestin expression in mouse hippocampal, olfactory bulb, striatum, and cortical tissue. Successful localization of these antigens was achieved using PFFP with and without antigen retrieval, with subsequent chromogenic DAB (3,3'-diaminobenzidine) development and immunofluorescence detection methods. The application of the PFFP in combination with methodologies of
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