ArticleJACS Au2023
Chemically Induced Extracellular Ice Nucleation Reduces Intracellular Ice Formation Enabling 2D and 3D Cellular Cryopreservation.
Article in JACS Au, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Chitosan and Chitosan Oligosaccharide Enhance Cryopreservation of Rabbit Testicular Tissue.Biology · 2026Article
- Development of an oscillating magnetic field-assisted supercooling system for bovine embryo preservation.Journal of animal science and technology · 2026Article
- Immersion Freezing Efficiency of ZnAlThe journal of physical chemistry. A · 2026Article
- Cryopreservation of Avian Erythrocytes Using Macromolecular Cryoprotectants.ACS polymers Au · 2026Article
- Size Effect of Stem Cell Spheroids in Their Cryopreservation Using Low-Molecular-Weight PEGs as a Cryoprotectant.Biomaterials research · 2026Article
- Induced Extracellular Ice Nucleation Protects Cocultured Spheroid Interior and Exterior during Cryopreservation.ACS biomaterials science & engineering · 2025Article
- Cryopreserved Kidney Epithelial (Vero) Cell Monolayers for Rapid Viral Quantification, Enabled by a Combination of Macromolecular Cryoprotectants.Biomacromolecules · 2024Article
- Low-Molecular-Weight PEGs for Cryopreservation of Stem Cell Spheroids.Biomaterials research · 2024Article
- Cryopreservation of assay-ready hepatocyte monolayers by chemically-induced ice nucleation: preservation of hepatic function and hepatotoxicity screening capabilities.Biomaterials science · 2023Article
- Advanced cryopreservation engineering strategies: the critical step to utilize stem cell products.Cell regeneration (London, England) · 2023Review
- Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation.Chemical communications (Cambridge, England) · 2023Article
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
3D cell assemblies such as spheroids reproduce the in vivo state more accurately than traditional 2D cell monolayers and are emerging as tools to reduce or replace animal testing. Current cryopreservation methods are not optimized for complex cell models, hence they are not easily banked and not as widely used as 2D models. Here we use soluble ice nucleating polysaccharides to nucleate extracellular ice and dramatically improve spheroid cryopreservation outcomes. This protects the cells beyond using DMSO alone, and with the major advantage that the nucleators function extracellularly and hence do not need to permeate the 3D cell models. Critical comparison of suspension, 2D and 3D cryopreservation outcomes demonstrated that warm-temperature ice nucleation reduces the formation of (fatal) intracellular ice, and in the case of 2/3D models this reduces propagation of ice between adjacent cells. This demonstrates that extracellular chemical nucleators could revolutionize the banking and deployment of advanced cell models.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.