Evidence map›Paper›PMID 37233188›Full record

ArticleJournal of cardiovascular development and disease2023

Rtf1 Transcriptionally Regulates Neonatal and Adult Cardiomyocyte Biology.

Adam D Langenbacher, Fei Lu, Lauren Crisman, Zi Yi Stephanie Huang, Douglas J Chapski, Thomas M Vondriska, Yibin Wang, Chen Gao, Jau-Nian Chen

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cardiovascular development and disease, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.5field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 2 countries.

Adam D LangenbacherDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90025, USA.ORCID 0000-0002-3752-0208
Fei LuDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90025, USA.
Lauren CrismanDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90025, USA.
Zi Yi Stephanie HuangDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90025, USA.
Douglas J ChapskiDepartments of Anesthesiology, Medicine, and Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90025, USA.ORCID 0000-0002-6730-7627
Thomas M VondriskaDepartments of Anesthesiology, Medicine, and Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90025, USA.
Yibin WangDepartments of Anesthesiology, Medicine, and Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90025, USA.
Chen GaoDepartments of Anesthesiology, Medicine, and Physiology, David Geffen School of Medicine, University of California, Los Angeles, CA 90025, USA.ORCID 0000-0001-7883-3270
Jau-Nian ChenDepartment of Molecular, Cell and Developmental Biology, University of California, Los Angeles, CA 90025, USA.ORCID 0000-0001-8807-3607
University of California, Los Angeles · US

Funding

Rtf1-dependent transcriptional regulation of heart developmentR01HL140472 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHEN, JAU-NIAN, TRINH, LE A. · 2018 to 2021
$1.6M
Impacts of transcription elongation on cardiac gene regulation during homeostasis and regenerationR01HL155905 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHEN, JAU-NIAN · 2021 to 2024
$1.6M
Genomic structural dynamics in fibroblasts during heart failureF32HL160099 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI CHAPSKI, DOUGLAS JOSEPH · 2022 to 2023
$100k
NHLBI NIH HHS F32 HL160099NHLBI NIH HHS R01 HL140472NHLBI NIH HHS R01 HL155905NIH HHS R01HL140472NIH HHS R01HL155905
6 · The paper itself

Abstract

The PAF1 complex component Rtf1 is an RNA Polymerase II-interacting transcription regulatory protein that promotes transcription elongation and the co-transcriptional monoubiquitination of histone 2B. Rtf1 plays an essential role in the specification of cardiac progenitors from the lateral plate mesoderm during early embryogenesis, but its requirement in mature cardiac cells is unknown. Here, we investigate the importance of Rtf1 in neonatal and adult cardiomyocytes using knockdown and knockout approaches. We demonstrate that loss of Rtf1 activity in neonatal cardiomyocytes disrupts cell morphology and results in a breakdown of sarcomeres. Similarly, Rtf1 ablation in mature cardiomyocytes of the adult mouse heart leads to myofibril disorganization, disrupted cell-cell junctions, fibrosis, and systolic dysfunction. Rtf1 knockout hearts eventually fail and exhibit structural and gene expression defects resembling dilated cardiomyopathy. Intriguingly, we observed that loss of Rtf1 activity causes a rapid change in the expression of key cardiac structural and functional genes in both neonatal and adult cardiomyocytes, suggesting that Rtf1 is continuously required to support expression of the cardiac gene program.

Indexed as

cardiomyocyteheartPAF1 complexRtf1transcription regulation

Identifiers

PMID37233188
PMCPMC10219292
OpenAlexW4377289915

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.