Evidence map›Paper›PMID 37232922›Full record

ArticleBiosensors2023

Development of an Efficient FRET-Based Ratiometric Uranium Biosensor.

Sandrine Sauge-Merle, Morgane Recuerda, Maria Rosa Beccia, David Lemaire, Rym Cherif, Nicolas Bremond, Fabienne Merola, Yasmina Bousmah, Catherine Berthomieu

Open access · goldAbstract read
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 70% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Sandrine Sauge-MerleAix Marseille Université, CEA, CNRS, BIAM, UMR7265, IPM, 13108 Saint Paul-Lez-Durance, France.ORCID 0000-0003-2721-3885
Morgane RecuerdaAix Marseille Université, CEA, CNRS, BIAM, UMR7265, IPM, 13108 Saint Paul-Lez-Durance, France.
Maria Rosa BecciaUniversité Côte d'Azur, CNRS, Institut de Chimie de Nice, UMR 7272, 06108 Nice, France.
David LemaireAix Marseille Université, CEA, CNRS, BIAM, UMR7265, IPM, 13108 Saint Paul-Lez-Durance, France.
Rym CherifAix Marseille Université, CEA, CNRS, BIAM, UMR7265, IPM, 13108 Saint Paul-Lez-Durance, France.
Nicolas BremondAix Marseille Université, CEA, CNRS, BIAM, UMR7265, IPM, 13108 Saint Paul-Lez-Durance, France.
Fabienne MerolaUniversité Paris-Saclay, CNRS, Institut de Chimie Physique, 91405 Orsay, France.
Yasmina BousmahUniversité Paris-Saclay, CNRS, Institut de Chimie Physique, 91405 Orsay, France.
Catherine BerthomieuAix Marseille Université, CEA, CNRS, BIAM, UMR7265, IPM, 13108 Saint Paul-Lez-Durance, France.ORCID 0000-0001-8784-5396
Centre National de la Recherche Scientifique · FR

Funding

Atomic Energy and Alternative Energies Commission Transversal Toxicology ProgramFrench National Centre for Scientific Research PF-Ressources of NEEDS Challenge
6 · The paper itself

Abstract

The dispersion of uranium in the environment can pose a problem for the health of humans and other living organisms. It is therefore important to monitor the bioavailable and hence toxic fraction of uranium in the environment, but no efficient measurement methods exist for this. Our study aims to fill this gap by developing a genetically encoded FRET-based ratiometric uranium biosensor. This biosensor was constructed by grafting two fluorescent proteins to both ends of calmodulin, a protein that binds four calcium ions. By modifying the metal-binding sites and the fluorescent proteins, several versions of the biosensor were generated and characterized

Indexed as

Biosensing TechniquesUraniumCalciumFluorescence Resonance Energy TransferGreen Fluorescent ProteinsHumansCalciumGreen Fluorescent ProteinsUraniumcalmodulinFRETgenetically encoded biosensormetal-bindingprotein engineeringselectivitysensingsensitivityuranium

Identifiers

PMID37232922
PMCPMC10216315
OpenAlexW4377115438

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.