Evidence map›Paper›PMID 37232905›Full record

ArticleBiosensors2023

Biomolecular Monitoring Tool Based on Lab-on-Chip for Virus Detection.

Francesca Costantini, Nicola Lovecchio, Manasa Nandimandalam, Ariana Manglli, Francesco Faggioli, Mara Biasin, Cesare Manetti, Pio Federico Roversi, Augusto Nascetti, Giampiero de Cesare and 1 more

Open access · goldAbstract read
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.5field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Francesca CostantiniCREA Research Centre for Plant Protection and Certification, 00156 Rome, Italy.ORCID 0000-0001-9576-8765
Nicola LovecchioDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0002-2571-2712
Manasa NandimandalamDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy.
Ariana ManglliCREA Research Centre for Plant Protection and Certification, 00156 Rome, Italy.
Francesco FaggioliCREA Research Centre for Plant Protection and Certification, 00156 Rome, Italy.ORCID 0000-0002-8395-1527
Mara BiasinDepartment of Biomedical and Clinical Sciences, University of Milan, Via G.B. Grassi, 20122 Milan, Italy.ORCID 0000-0003-3671-4235
Cesare ManettiDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy.
Pio Federico RoversiCREA Research Centre for Plant Protection and Certification, 00156 Rome, Italy.ORCID 0000-0002-8098-8367
Augusto NascettiSchool of Aerospace Engineering, Sapienza University of Rome, 00138 Rome, Italy.ORCID 0000-0001-8138-7494
Giampiero de CesareDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0002-9935-2975
Domenico CaputoDepartment of Environmental Biology, Sapienza University of Rome, 00185 Rome, Italy.ORCID 0000-0002-3709-6662
Sapienza University of Rome · ITInstitute for Sustainable Plant Protection · ITUniversity of Milan · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Lab-on-Chip (LoC) devices for performing real-time PCR are advantageous compared to standard equipment since these systems allow to conduct in-field quick analysis. The development of LoCs, where the components for performing the nucleic acid amplification are all integrated, can be an issue. In this work, we present a LoC-PCR device where thermalization, temperature control and detection elements are all integrated on a single glass substrate named System-on-Glass (SoG) obtained using metal thin-film deposition. By using a microwell plate optically coupled with the SoG, real-time reverse transcriptase PCR of RNA extracted from both a plant and human virus has been carried out in the developed LoC-PCR device. The limit of detection and time of analysis for the detection of the two viruses by using the LoC-PCR were compared with those achieved by standard equipment. The results showed that the two systems can detect the same concentration of RNA; however, the LoC-PCR performs the analysis in half of the time compared to the standard thermocycler, with the advantage of the portability, leading to a point-of-care device for several diagnostic applications.

Indexed as

Lab-On-A-Chip DevicesVirusesHumansNucleic Acid Amplification TechniquesReal-Time Polymerase Chain ReactionRNA, ViralRNA, ViralLab-on-Chipreal-time RT-PCRSystem-on-Glassvirus detection

Identifiers

PMID37232905
PMCPMC10216243
OpenAlexW4378347528

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.