Evidence map›Paper›PMID 37232879›Full record

ArticleBiosensors2023

On-Chip Nucleic Acid Purification Followed by ddPCR for SARS-CoV-2 Detection.

Cong Ma, Yimeng Sun, Yuhang Huang, Zehang Gao, Yaru Huang, Ikshu Pandey, Chunping Jia, Shilun Feng, Jianlong Zhao

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 30% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 2 countries.

Cong MaSchool of Information Science and Technology, ShanghaiTech University, Shanghai 201210, China.ORCID 0000-0003-0433-3243
Yimeng SunState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Yuhang HuangState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Zehang GaoState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.ORCID 0000-0002-6410-6440
Yaru HuangState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Ikshu PandeyDepartment of Materials Science and Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
Chunping JiaState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Shilun FengState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.ORCID 0000-0002-2560-2417
Jianlong ZhaoState Key Laboratory of Transducer Technology, Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai 200050, China.
Chinese Academy of Sciences · CNJohns Hopkins University · US

Funding

Shanghai Pujiang Program 21PJ1415000the equipment research and development projects of the Chinese Academy of Sciences GJJSTD20210006the equipment research and development projects of the Chinese Academy of Sciences YJKYYQ20210049the Science and Technology Commission of Shanghai Municipality 22xtcx00100
6 · The paper itself

Abstract

We developed a microfluidic chip integrated with nucleic acid purification and droplet-based digital polymerase chain reaction (ddPCR) modules to realize a 'sample-in, result-out' infectious virus diagnosis. The whole process involved pulling magnetic beads through drops in an oil-enclosed environment. The purified nucleic acids were dispensed into microdroplets by a concentric-ring, oil-water-mixing, flow-focusing droplets generator driven under negative pressure conditions. Microdroplets were generated with good uniformity (CV = 5.8%), adjustable diameters (50-200 μm), and controllable flow rates (0-0.3 μL/s). Further verification was provided by quantitative detection of plasmids. We observed a linear correlation of R

Indexed as

COVID-19Nucleic AcidsHumansOligonucleotide Array Sequence AnalysisPolymerase Chain ReactionSARS-CoV-2Nucleic AcidsddPCRdroplet generationnucleic acid purificationSARS-CoV-2

Identifiers

PMID37232879
PMCPMC10216157
OpenAlexW4378348023

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.