ArticleNature biotechnology2024
Sequencing by avidity enables high accuracy with low reagent consumption.
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Article in Nature biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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50citing papers in PubMed
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50 citing papers in PubMed.
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- Intrahost mutational dynamics parallel long-term genome evolution in endosymbionts.Molecular biology and evolution · 2026Article
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- Benchmarking Q40 sequencing for sensitive and efficient detection of rare genomic variants.Genome biology · 2026Article
- Whole-genome sequencing with AVITI and NovaSeq X Plus reveals comparable performance with contextual biases.NAR genomics and bioinformatics · 2026Article
- A family portrait of the genomic factors shaping tandem repeat mutagenesis.bioRxiv : the preprint server for biology · 2026Article
- A complete human pancreatic cancer genome.bioRxiv : the preprint server for biology · 2026Article
- AVITI sequencing of a four-generation CEPH/Utah pedigree confirms low mutation rates at homopolymer loci despite their low sequence complexity.Genome biology · 2026Article
- Characterization of subclonal variants in HG002 Genome in a Bottle reference material as a resource for benchmarking variant callers.Cell genomics · 2026Article
- Application of SNV Detection Methods for Market Control of Food Products from New Genomic Techniques.International journal of molecular sciences · 2026Review
- Evaluation of ensilication technology for ambient DNA preservation.NAR molecular medicine · 2026Article
- Engineering the poly(A) tail for therapeutic mRNA: from expression control to manufacturing robustness.Frontiers in bioengineering and biotechnology · 2026Review
- Advances in Massive Parallel Sequencing: From Genomics to Spatial Transcriptomics.Advances in experimental medicine and biology · 2026Review
- Constellation illuminates rare disease genetics.medRxiv : the preprint server for health sciences · 2025Article
- AVITI sequencing of a four-generation CEPH/Utah pedigree confirms low mutation rates at homopolymer loci despite their low sequence complexity.bioRxiv : the preprint server for biology · 2025Article
- A simplified hybrid capture approach retains high specificity and enables PCR-free workflow.BMC genomics · 2025Article
- Accurate human genome analysis with element avidity sequencing.BMC bioinformatics · 2025Article
- Development and extensive sequencing of a broadly-consented Genome in a Bottle matched tumor-normal pair.Scientific data · 2025Article
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Authors and funding
139 authors.
Sinan Arslan *Element Biosciences, San Diego, CA, USA.
Francisco J Garcia *Element Biosciences, San Diego, CA, USA.
Minghao Guo *Element Biosciences, San Diego, CA, USA.
Matthew W Kellinger *Element Biosciences, San Diego, CA, USA.
Semyon Kruglyak *Element Biosciences, San Diego, CA, USA.
Jake A LeVieux *Element Biosciences, San Diego, CA, USA.
Adeline H Mah *Element Biosciences, San Diego, CA, USA.
Haosen Wang *Element Biosciences, San Diego, CA, USA.
Junhua Zhao *Element Biosciences, San Diego, CA, USA.
Chunhong Zhou *Element Biosciences, San Diego, CA, USA.
Andrew AltomareElement Biosciences, San Diego, CA, USA.
John BaileyElement Biosciences, San Diego, CA, USA.
Matthew B ByrneElement Biosciences, San Diego, CA, USA.
Chiting ChangElement Biosciences, San Diego, CA, USA.
Steve X ChenElement Biosciences, San Diego, CA, USA.
Byungrae ChoElement Biosciences, San Diego, CA, USA.
Claudia N DennlerElement Biosciences, San Diego, CA, USA.
Vivian T DienElement Biosciences, San Diego, CA, USA.ORCID http://orcid.org/0000-0003-3237-4325
Derek FullerElement Biosciences, San Diego, CA, USA.
Ryan KelleyElement Biosciences, San Diego, CA, USA.
Omid KhandanElement Biosciences, San Diego, CA, USA.
Michael G KleinElement Biosciences, San Diego, CA, USA.
Michael KimElement Biosciences, San Diego, CA, USA.
Bryan R LajoieElement Biosciences, San Diego, CA, USA.
Bill LinElement Biosciences, San Diego, CA, USA.
Yu LiuElement Biosciences, San Diego, CA, USA.
Tyler LopezElement Biosciences, San Diego, CA, USA.
Peter T MainsElement Biosciences, San Diego, CA, USA.
Andrew D PriceElement Biosciences, San Diego, CA, USA.
Samantha R RobertsonElement Biosciences, San Diego, CA, USA.
Hermes Taylor-WeinerElement Biosciences, San Diego, CA, USA.
Ramreddy TippanaElement Biosciences, San Diego, CA, USA.
Austin B TomaneyElement Biosciences, San Diego, CA, USA.
Su ZhangElement Biosciences, San Diego, CA, USA.
Minna AbtahiElement Biosciences, San Diego, CA, USA.
Mark R AmbrosoElement Biosciences, San Diego, CA, USA.
Rosita BajariElement Biosciences, San Diego, CA, USA.ORCID http://orcid.org/0000-0002-1402-7496
Ava M BellizziElement Biosciences, San Diego, CA, USA.
Chris B BenitezElement Biosciences, San Diego, CA, USA.
Daniel R BerardElement Biosciences, San Diego, CA, USA.
Lorenzo BertiElement Biosciences, San Diego, CA, USA.
Kelly N BleaseElement Biosciences, San Diego, CA, USA.
Angela P BlumElement Biosciences, San Diego, CA, USA.
Andrew M BoddickerElement Biosciences, San Diego, CA, USA.
Leo BondarElement Biosciences, San Diego, CA, USA.
Chris BrownElement Biosciences, San Diego, CA, USA.
Chris A BuiElement Biosciences, San Diego, CA, USA.
Juan Calleja-AguirreElement Biosciences, San Diego, CA, USA.
Kevin CappaElement Biosciences, San Diego, CA, USA.
Joshua ChanElement Biosciences, San Diego, CA, USA.
Victor W ChangElement Biosciences, San Diego, CA, USA.
Katherine CharovElement Biosciences, San Diego, CA, USA.
Xiyi ChenElement Biosciences, San Diego, CA, USA.
Rodger M ConstandseElement Biosciences, San Diego, CA, USA.
Weston DamronElement Biosciences, San Diego, CA, USA.
Mariam DawoodElement Biosciences, San Diego, CA, USA.
Nicole DeBuonoElement Biosciences, San Diego, CA, USA.
John D DimalantaElement Biosciences, San Diego, CA, USA.
Laure EdoliElement Biosciences, San Diego, CA, USA.
Keerthana ElangoElement Biosciences, San Diego, CA, USA.
Nikka FaustinoElement Biosciences, San Diego, CA, USA.
Chao FengElement Biosciences, San Diego, CA, USA.
Matthew FerrariElement Biosciences, San Diego, CA, USA.
Keith FrankieElement Biosciences, San Diego, CA, USA.
Adam FriesElement Biosciences, San Diego, CA, USA.
Anne GallowayElement Biosciences, San Diego, CA, USA.
Vlad GavrilaElement Biosciences, San Diego, CA, USA.
Gregory J GemmenElement Biosciences, San Diego, CA, USA.
James GhadialiElement Biosciences, San Diego, CA, USA.
Arash GhorbaniElement Biosciences, San Diego, CA, USA.
Logan A GoddardElement Biosciences, San Diego, CA, USA.
Adriana Roginski GuetterElement Biosciences, San Diego, CA, USA.
Garren L HendricksElement Biosciences, San Diego, CA, USA.
Jendrik HentschelElement Biosciences, San Diego, CA, USA.
Daniel J HonigfortElement Biosciences, San Diego, CA, USA.
Yun-Ting HsiehElement Biosciences, San Diego, CA, USA.
Yu-Hsien Hwang FuElement Biosciences, San Diego, CA, USA.
Scott K ImElement Biosciences, San Diego, CA, USA.
Chaoyi JinElement Biosciences, San Diego, CA, USA.
Shradha KabuElement Biosciences, San Diego, CA, USA.
Daniel E KincadeElement Biosciences, San Diego, CA, USA.
Shawn LevyElement Biosciences, San Diego, CA, USA.
Yu LiElement Biosciences, San Diego, CA, USA.
Vincent K LiangElement Biosciences, San Diego, CA, USA.
William H LightElement Biosciences, San Diego, CA, USA.
Jonathan B LipsherElement Biosciences, San Diego, CA, USA.
Tsung-Li LiuElement Biosciences, San Diego, CA, USA.
Grace LongElement Biosciences, San Diego, CA, USA.
Rui MaElement Biosciences, San Diego, CA, USA.
John M MaillouxElement Biosciences, San Diego, CA, USA.
Kyle A MandlaElement Biosciences, San Diego, CA, USA.
Anyssa R MartinezElement Biosciences, San Diego, CA, USA.
Max MassElement Biosciences, San Diego, CA, USA.
Daniel T McKeanElement Biosciences, San Diego, CA, USA.
Michael MeronElement Biosciences, San Diego, CA, USA.
Edmund A MillerElement Biosciences, San Diego, CA, USA.
Celyne S MohElement Biosciences, San Diego, CA, USA.
Rachel K MooreElement Biosciences, San Diego, CA, USA.
Juan MorenoElement Biosciences, San Diego, CA, USA.
Jordan M NeysmithElement Biosciences, San Diego, CA, USA.
Cassandra S NimanElement Biosciences, San Diego, CA, USA.
Jesus M NunezElement Biosciences, San Diego, CA, USA.
Micah T OjedaElement Biosciences, San Diego, CA, USA.
Sara Espinosa OrtizElement Biosciences, San Diego, CA, USA.
Jenna OwensElement Biosciences, San Diego, CA, USA.
Geoffrey PilandElement Biosciences, San Diego, CA, USA.
Daniel J ProctorElement Biosciences, San Diego, CA, USA.
Josua B PurbaElement Biosciences, San Diego, CA, USA.
Michael RayElement Biosciences, San Diego, CA, USA.
Daisong RongElement Biosciences, San Diego, CA, USA.
Virginia M SaadeElement Biosciences, San Diego, CA, USA.
Sanchari SahaElement Biosciences, San Diego, CA, USA.
Gustav Santo TomasElement Biosciences, San Diego, CA, USA.
Nicholas ScheidlerElement Biosciences, San Diego, CA, USA.
Luqmanal H SirajudeenElement Biosciences, San Diego, CA, USA.
Samantha SnowElement Biosciences, San Diego, CA, USA.
Gudrun StengelElement Biosciences, San Diego, CA, USA.
Ryan StinsonElement Biosciences, San Diego, CA, USA.
Michael J StoneElement Biosciences, San Diego, CA, USA.
Keoni J SundsethElement Biosciences, San Diego, CA, USA.
Eileen ThaiElement Biosciences, San Diego, CA, USA.
Connor J ThompsonElement Biosciences, San Diego, CA, USA.
Marco TjioeElement Biosciences, San Diego, CA, USA.
Christy L TrejoElement Biosciences, San Diego, CA, USA.
Greg TriegerElement Biosciences, San Diego, CA, USA.
Diane Ni TruongElement Biosciences, San Diego, CA, USA.
Ben TseElement Biosciences, San Diego, CA, USA.
Benjamin VoilesElement Biosciences, San Diego, CA, USA.
Henry VuongElement Biosciences, San Diego, CA, USA.
Jennifer C WongElement Biosciences, San Diego, CA, USA.
Chiung-Ting WuElement Biosciences, San Diego, CA, USA.
Hua YuElement Biosciences, San Diego, CA, USA.
Yingxian YuElement Biosciences, San Diego, CA, USA.
Ming YuElement Biosciences, San Diego, CA, USA.
Xi ZhangElement Biosciences, San Diego, CA, USA.
Da ZhaoElement Biosciences, San Diego, CA, USA.ORCID http://orcid.org/0000-0002-8292-5036
Genhua ZhengElement Biosciences, San Diego, CA, USA.
Molly HeElement Biosciences, San Diego, CA, USA.
Michael PreviteElement Biosciences, San Diego, CA, USA. mprevite@elembio.com.ORCID http://orcid.org/0009-0004-0533-6280
Funding
No grant is acknowledged in the PubMed record.
Abstract
We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer-nucleotide complexes bound to clonal copies of DNA targets. These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides from micromolar to nanomolar and yield negligible dissociation rates. Avidity sequencing achieves high accuracy, with 96.2% and 85.4% of base calls having an average of one error per 1,000 and 10,000 base pairs, respectively. We show that the average error rate of avidity sequencing remained stable following a long homopolymer.
Indexed as
DNANucleotidesBase PairingDNA ReplicationPolymersDNANucleotidesPolymers
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