Evidence map›Paper›PMID 37229274›Full record

ArticleJournal of biomedical optics2023

Imaging the rotational mobility of carbon dot-gold nanoparticle conjugates using frequency domain wide-field time-resolved fluorescence anisotropy.

Gilad Yahav, Shweta Pawar, Yitzchak Weber, Bar Atuar, Hamootal Duadi, Dror Fixler

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biomedical optics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Probing Polarity and pH Sensitivity of Carbon Dots inNanomaterials (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Gilad YahavBar Ilan University, The Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Ramat Gan, Israel.ORCID 0009-0002-4824-4752
Shweta PawarBar Ilan University, The Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Ramat Gan, Israel.
Yitzchak WeberBar Ilan University, The Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Ramat Gan, Israel.ORCID 0000-0003-3551-3343
Bar AtuarBar Ilan University, The Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Ramat Gan, Israel.
Hamootal DuadiBar Ilan University, The Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Ramat Gan, Israel.
Dror FixlerBar Ilan University, The Faculty of Engineering and the Institute of Nanotechnology and Advanced Materials, Ramat Gan, Israel.ORCID 0000-0003-0963-7908

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Significance: Wide-field measurements of time-resolved fluorescence anisotropy (TR-FA) provide pixel-by-pixel information about the rotational mobility of fluorophores, reflecting changes in the local microviscosity and other factors influencing the fluorophore's diffusional motion. These features offer promising potential in many research fields, including cellular imaging and biochemical sensing, as demonstrated by previous works. Nevertheless, Aim: To extend existing frequency domain (FD) fluorescence lifetime (FLT) imaging microscopy (FLIM) to FD TR-FA imaging (TR-FAIM), which produces visual maps of the FLT and Approach: The proof of concept of the combined FD FLIM/ FD TR-FAIM was validated on seven fluorescein solutions with increasing viscosities and was applied for comprehensive study of two types of CD-gold nano conjugates. Results: The FLT of fluorescein samples was found to decrease from Conclusions: Through the combined FD FLIM/ FD TR-FAIM, a large variety of information can be probed (FI, FLT,

Indexed as

GoldMetal NanoparticlesFluoresceinFluorescence PolarizationFluorescent DyesFluoresceinFluorescent DyesGoldfluorescence anisotropyfluorescence anisotropy decayfluorescence lifetime imaging microscopyfluorescent lifetimefrequency domainrotational correlation time

Identifiers

PMID37229274
PMCPMC10203731

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.