ArticleIranian journal of biotechnology2023
Response Surface Methodology to Optimize the Expression Efficiency of Recombinant Reteplase.
Article in Iranian journal of biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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4 citing papers in PubMed, 3 citations in OpenAlex.
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Authors and funding
7 authors at 2 institutions in 1 country.
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Abstract
Background: Over expression of Reteplase enzyme has already been studies in the periplasmic space of Objectives: Optical cell density (OD), IPTG concentration, and expression time are highly effective in the protein expression rates. Therefore, we aimed to determine the optimum levels of these factors for reteplase expression using response surface methodology (RSM). Materials and Methods: The pET21b plasmid was used to sub-clone the designed reteplase gene. Then, the gene was transformed into Results: Sequence optimization removed all undesirable sequences of the designed gene. Transformation into Conclusion: The obtained results indicate that IPTG concentration, OD, and expression time are significantly involved in the augmentation of recombinant reteplase expression. To the best of our knowledge, this is the first study to assess the combined effect of these factors on reteplase expression. Further RSM-based experiments would bring about new insights regarding the best conditions for reteplase expression.
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