Evidence map›Paper›PMID 37226739›Full record

ArticleAnimal biotechnology2023

Diverse pathogen-associated molecular patterns affect transcription of genes in the toll-like receptor signaling pathway in goat blood.

Kingsley Ekwemalor, Emmanuel Asiamah, Sarah Adjei-Fremah, Eboghoye Eluka-Okoludoh, Bharath Mulakala, Bertha Osei, Mulumebet Worku

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.7field-weighted citation impact, top 28% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 4 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Kingsley EkwemalorDepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USA.
Emmanuel AsiamahDepartment of Agriculture, University of Arkansas at Pine Bluff, Pine Bluff, AR, USA.
Sarah Adjei-FremahDepartment of Biological Sciences, Winston-Salem State University, Winston-Salem, NC, USA.
Eboghoye Eluka-OkoludohDepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USA.
Bharath MulakalaDepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USA.
Bertha OseiDepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USA.
Mulumebet WorkuDepartment of Animal Sciences, North Carolina Agricultural and Technical State University, Greensboro, NC, USA.
North Carolina Agricultural and Technical State University · USUniversity of Arkansas at Pine Bluff · USWinston-Salem State University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Pathogen-associated molecular patterns (PAMPs) such as lipopolysaccharide (LPS), peptidoglycan (PGN), Polyinosinic-polycytidylic acid (poly I:C), and CpG Oligodeoxynucleotides (ODN) are recognized by Toll-like receptors (TLR). This study aimed to investigate the effect of diverse PAMPs on the transcription of TLR signaling pathway genes in goat blood. Whole blood was collected from 3 female BoerXSpanish goats and treated with the following PAMPs: 10 µg/ml LPS, PGN, CpG ODN (2216), CpG ODN (2006), and 12.5 µg/ml Poly I:C. Blood-treated PBS served as a control. The expression of 84 genes in the human TLR signaling pathway RT2 PCR Array (Qiagen) was evaluated using real-time PCR. Treatment with PBS affected the expression of 74 genes, Poly I:C affected the expression of 40 genes, t ODN 2006 affected the expression of 50 genes, ODN 2216 affected the expression of 52 genes, LPS affected the expression of 49 genes, while PGN affected the expression of 49 genes. Our results show that PAMPs modulated and increased the expression of genes in the TLR signaling pathway. These results highlight important insights into how the host responds to different pathogens and may help design adjuvants for therapeutics and vaccines that target different.

Indexed as

LipopolysaccharidesPathogen-Associated Molecular Pattern MoleculesAnimalsFemaleGoatsHumansPoly ISignal TransductionToll-Like ReceptorsLipopolysaccharidesPathogen-Associated Molecular Pattern MoleculesPoly IToll-Like Receptorsgene expressionLPSPAMPpeptidoglycan PGNpoly ICToll-like receptors

Identifiers

PMID37226739
PMCPMC13353395
OpenAlexW4378173567

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.