ArticleNational science review2023
Dynamics of synthetic yeast chromosome evolution shaped by hierarchical chromatin organization.
Article in National science review, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- High-throughput editing boosts the construction of next-generation microbial cell factories.Synthetic and systems biotechnology · 2026Review
- Synthetic rewriting of the IGH locus.Cell genomics · 2026Article
- Synthetic rewriting technologies in mammalian cells.Nature communications · 2026Review
- Advances in microbial extracellular vesicles: synthetic biology platforms and medical applications.Extracellular vesicles and circulating nucleic acids · 2026Review
- Assembly and delivery of large DNA via chromosome elimination in yeast.Nature protocols · 2025Review
- Design and structure of overlapping regions in PCA via deep learning.Synthetic and systems biotechnology · 2025Article
- Multiplex generation and single-cell analysis of structural variants in mammalian genomes.Science (New York, N.Y.) · 2025Article
- Construction and iterative redesign of synXVI a 903 kb synthetic Saccharomyces cerevisiae chromosome.Nature communications · 2025Article
- Identifying associations between short tandem repeat sequences and gene expression in yeast reveals specific repeated motifs encoding transcriptional regulatory proteins.Computational and structural biotechnology journal · 2025Article
- Towards the first synthetic eukaryotic cell.Biosafety and health · 2024Review
- Enhancing whole yeast genome rearrangements through multiple LoxPsym sequences.Science China. Life sciences · 2024Article
- An Optimized Genotyping Workflow for Identifying Highly SCRaMbLEd Synthetic Yeasts.ACS synthetic biology · 2024Article
- Multiplex generation and single cell analysis of structural variants in a mammalian genome.bioRxiv : the preprint server for biology · 2024Article
- Large-scale genomic rearrangements boost SCRaMbLE in Saccharomyces cerevisiae.Nature communications · 2024Article
- Application and Technical Challenges in Design, Cloning, and Transfer of Large DNA.Bioengineering (Basel, Switzerland) · 2023Review
- Investigation of Genome Biology by Synthetic Genome Engineering.Bioengineering (Basel, Switzerland) · 2023Review
- Systematic dissection of key factors governing recombination outcomes by GCE-SCRaMbLE.Nature communications · 2022Article
- Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE.Synthetic and systems biotechnology · 2022Article
- Recent Advances in Directed Yeast Genome Evolution.Journal of fungi (Basel, Switzerland) · 2022Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic genome evolution provides a dynamic approach for systematically and straightforwardly exploring evolutionary processes. Synthetic Chromosome Rearrangement and Modification by LoxP-mediated Evolution (SCRaMbLE) is an evolutionary system intrinsic to the synthetic yeast genome that can rapidly drive structural variations. Here, we detect over 260 000 rearrangement events after the SCRaMbLEing of a yeast strain harboring 5.5 synthetic yeast chromosomes (synII, synIII, synV, circular synVI, synIXR and synX). Remarkably, we find that the rearrangement events exhibit a specific landscape of frequency. We further reveal that the landscape is shaped by the combined effects of chromatin accessibility and spatial contact probability. The rearrangements tend to occur in 3D spatially proximal and chromatin-accessible regions. The enormous numbers of rearrangements mediated by SCRaMbLE provide a driving force to potentiate directed genome evolution, and the investigation of the rearrangement landscape offers mechanistic insights into the dynamics of genome evolution.
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