ArticleThe Journal of cell biology1986
Microtubules and microtubule-associated proteins from the nematode Caenorhabditis elegans: periodic cross-links connect microtubules in vitro.
Article in The Journal of cell biology, 1986. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 56 citations in OpenAlex.
- Next-generation immunotherapeutic approaches for blood cancers: Exploring the efficacy of CAR-T and cancer vaccines.Experimental hematology & oncology · 2025Review
- A microtubule bestiary: structural diversity in tubulin polymers.Molecular biology of the cell · 2017Review
- Role of tau in the spatial organization of axonal microtubules: keeping parallel microtubules evenly distributed despite macromolecular crowding.Cellular and molecular life sciences : CMLS · 2016Article
- Mechanical Effects of Dynamic Binding between Tau Proteins on Microtubules during Axonal Injury.Biophysical journal · 2015Article
- Tubulin acetylation: responsible enzymes, biological functions and human diseases.Cellular and molecular life sciences : CMLS · 2015Review
- Laulimalide induces dose-dependent modulation of microtubule behaviour in the C. elegans embryo.PloS one · 2013Article
- Genetically separable functions of the MEC-17 tubulin acetyltransferase affect microtubule organization.Current biology : CB · 2012Article
- Complementary dimerization of microtubule-associated tau protein: Implications for microtubule bundling and tau-mediated pathogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- Two heteromeric kinesin complexes in chemosensory neurons and sensory cilia of Caenorhabditis elegans.Molecular biology of the cell · 1999Article
- Lattice defects in microtubules: protofilament numbers vary within individual microtubules.The Journal of cell biology · 1992Article
- The isolation and in situ location of adligin: the microtubule cross-linking protein from Caenorhabditis elegans.The Journal of cell biology · 1989Article
- A 60-kDa cytoskeletal protein from Trypanosoma brucei brucei can interact with membranes and with microtubules.Proceedings of the National Academy of Sciences of the United States of America · 1988Article
- Periodic crosslinking of microtubules by cytoplasmic microtubule-associated and microtubule-corset proteins from a trypanosomatid.Proceedings of the National Academy of Sciences of the United States of America · 1987Article
- Molecular cloning and three-dimensional structure prediction of a novel alpha-tubulin in Caenorhabditis elegans.Journal of cellular and molecular medicineArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
The nematode Caenorhabditis elegans should be an excellent model system in which to study the role of microtubules in mitosis, embryogenesis, morphogenesis, and nerve function. It may be studied by the use of biochemical, genetic, molecular biological, and cell biological approaches. We have purified microtubules and microtubule-associated proteins (MAPs) from C. elegans by the use of the anti-tumor drug taxol (Vallee, R. B., 1982, J. Cell Biol., 92:435-44). Approximately 0.2 mg of microtubules and 0.03 mg of MAPs were isolated from each gram of C. elegans. The C. elegans microtubules were smaller in diameter than bovine microtubules assembled in vitro in the same buffer. They contained primarily 9-11 protofilaments, while the bovine microtubules contained 13 protofilaments. The principal MAP had an apparent molecular weight of 32,000 and the minor MAPs were 30,000, 45,000, 47,000, 50,000, 57,000, and 100,000-110,000 mol wt as determined by SDS-gel electrophoresis. The microtubules were observed, by electron microscopy of negatively stained preparations, to be connected by stretches of highly periodic cross-links. The cross-links connected the adjacent protofilaments of aligned microtubules, and occurred at a frequency of one cross-link every 7.7 +/- 0.9 nm, or one cross-link per tubulin dimer along the protofilament. The cross-links were removed when the MAPs were extracted from the microtubules with 0.4 M NaCl. The cross-links then re-formed when the microtubules and the MAPs were recombined in a low salt buffer. These results strongly suggest that the cross-links are composed of MAPs.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.