Evidence map›Paper›PMID 37221550›Full record

ArticleJournal of ovarian research2023

Extracellular vesicle-microRNAs mediated response of bovine ovaries to seasonal environmental changes.

Ahmed Gad, Kamryn Joyce, Nico Graham Menjivar, Daniella Heredia, Camila Santos Rojas, Dawit Tesfaye, Angela Gonella-Diaza

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
2.9field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Ahmed GadDepartment of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
Kamryn JoyceNorth Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Marianna, FL, USA.
Nico Graham MenjivarDepartment of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA.
Daniella HerediaNorth Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Marianna, FL, USA.
Camila Santos RojasNorth Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Marianna, FL, USA.
Dawit TesfayeDepartment of Biomedical Sciences, Animal Reproduction and Biotechnology Laboratory (ARBL), Department of Biomedical Sciences, Colorado State University, Fort Collins, CO, USA. dawit.tesfaye@colostate.edu.
Angela Gonella-DiazaNorth Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, Marianna, FL, USA.
Southwest Florida Research · USColorado State University · USCairo University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundAmong the various seasonal environmental changes, elevated ambient temperature during the summer season is a main cause of stress in dairy and beef cows, leading to impaired reproductive function and fertility. Follicular fluid extracellular vesicles (FF-EVs) play an important role in intrafollicular cellular communication by, in part, mediating the deleterious effects of heat stress (HS). Here we aimed to investigate the changes in FF-EV miRNA cargoes in beef cows in response to seasonal changes: summer (SUM) compared to the winter (WIN) season using high throughput sequencing of FF-EV-coupled miRNAs. In addition to their biological relevance, the potential mechanisms involved in the packaging and release of those miRNAs as a response to environmental HS were elucidated.

resultsSequencing analysis revealed that an average of 6.6% of the EV-RNA mapped reads were annotated to bovine miRNAs. Interestingly, miR-148a, miR-99a-5p, miR-10b, and miR-143 were the top four miRNAs in both groups accounting for approximately 52 and 62% of the total miRNA sequence reads in the SUM and WIN groups, respectively. A group of 16 miRNAs was up-regulated and 8 miRNAs were down-regulated in the SUM compared to the WIN group. Five DE-miRNAs (miR-10a, miR-10b, miR-26a, let-7f, and miR-1246) were among the top 20 expressed miRNA lists. Sequence motif analysis revealed the appearance of two specific motifs in 13 out of the 16 upregulated miRNAs under HS conditions. Both motifs were found to be potentially bonded by specific RNA binding proteins including Y-box binding proteins (YBX1 and YBX2) and RBM42.

conclusionOur findings indicate that FF EV-coupled miRNA profile varies under seasonal changes. These miRNAs could be a good indicator of the cellular mechanism in mediating HS response and the potential interplay between miRNA motifs and RNA binding proteins can be one of the mechanisms governing the packaging and release of miRNAs via EVs to facilitate cellular survival.

Indexed as

Extracellular VesiclesMicroRNAsAnimalsCattleCell CommunicationFemaleOvarySeasonsMicroRNAsBeef cowsExtracellular vesiclesFollicular fluidHeat stressmiRNA

Identifiers

PMID37221550
PMCPMC10207814
OpenAlexW4377820053

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.