Evidence map›Paper›PMID 37220748›Full record

ArticleDevelopmental cell2023

Actuation of single downstream nodes in growth factor network steers immune cell migration.

Dhiman Sankar Pal, Tatsat Banerjee, Yiyan Lin, Félix de Trogoff, Jane Borleis, Pablo A Iglesias, Peter N Devreotes

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Developmental cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
7.5field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed, 31 citations in OpenAlex.

  1. Article
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  7. Review
  8. Article
  9. Signaling and actin waves at a glance.Journal of cell science · 2025
    Review
  10. Article
  11. Article
  12. Ras-mediated dynamic and biphasic regulation of cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  13. Article
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  15. Article
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  17. Article
  18. Making PI3K superfamily enzymes run faster.Advances in biological regulation · 2025
    Review
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 2 countries.

Dhiman Sankar PalDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA. Electronic address: dhimanpal8@gmail.com.
Tatsat BanerjeeDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA; Department of Chemical and Biomolecular Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, USA.
Yiyan LinDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA; Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Félix de TrogoffDepartment of Mechanical Engineering, STI School of Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland; Department of Electrical and Computer Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, USA.
Jane BorleisDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA.
Pablo A IglesiasDepartment of Electrical and Computer Engineering, Whiting School of Engineering, Johns Hopkins University, Baltimore, MD, USA.
Peter N DevreotesDepartment of Cell Biology and Center for Cell Dynamics, School of Medicine, Johns Hopkins University, Baltimore, MD, USA; Department of Biological Chemistry, School of Medicine, Johns Hopkins University, Baltimore, MD, USA. Electronic address: pnd@jhmi.edu.
Johns Hopkins University · US

Funding

Excitable Networks in Directed Cell MigrationR35GM118177 · NIGMS · JOHNS HOPKINS UNIVERSITY · PI Peter N Devreotes · 2016 to 2026
$12.1M
Zeiss LSM780 Confocal Microscope for a Core FacilityS10OD016374 · OD · JOHNS HOPKINS UNIVERSITY · PI KUO, SCOT CHARLES · 2013 to 2013
$584k
NIGMS NIH HHS R35 GM118177NIH HHS S10 OD016374
6 · The paper itself

Abstract

Ras signaling is typically associated with cell growth, but not direct regulation of motility or polarity. By optogenetically targeting different nodes in the Ras/PI3K/Akt network in differentiated human HL-60 neutrophils, we abruptly altered protrusive activity, bypassing the chemoattractant receptor/G-protein network. First, global recruitment of active KRas4B/HRas isoforms or a RasGEF, RasGRP4, immediately increased spreading and random motility. Second, activating Ras at the cell rear generated new protrusions, reversed pre-existing polarity, and steered sustained migration in neutrophils or murine RAW 264.7 macrophages. Third, recruiting a RasGAP, RASAL3, to cell fronts extinguished protrusions and changed migration direction. Remarkably, persistent RASAL3 recruitment at stable fronts abrogated directed migration in three different chemoattractant gradients. Fourth, local recruitment of the Ras-mTORC2 effector, Akt, in neutrophils or Dictyostelium amoebae generated new protrusions and rearranged pre-existing polarity. Overall, these optogenetic effects were mTORC2-dependent but relatively independent of PI3K. Thus, receptor-independent, local activations of classical growth-control pathways directly control actin assembly, cell shape, and migration modes.

Indexed as

DictyosteliumProto-Oncogene Proteins c-aktAnimalsCell MovementHumansIntercellular Signaling Peptides and ProteinsMechanistic Target of Rapamycin Complex 2MicePhosphatidylinositol 3-KinasesIntercellular Signaling Peptides and ProteinsMechanistic Target of Rapamycin Complex 2Phosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktactin cytoskeletonbiochemical excitabilitycancerdevelopmentimmunityimmunotherapyleukocytesmetastasismTOR signalingoptogenetics

Identifiers

PMID37220748
PMCPMC10524337
OpenAlexW4377246555

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.