Evidence map›Paper›PMID 37219668›Full record

ArticleMolecular biology reports2023

Deciphering the level of genetic diversity in some aegilops species using CAAT box-derived polymorphism (CBDP) and start codon target polymorphism (SCoT) markers.

Ali Sajjad Bokaei, Omid Sofalian, Behzad Sorkhilalehloo, Ali Asghari, Alireza Pour-Aboughadareh

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Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

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Ali Sajjad BokaeiDepartment of Agronomy and Plant Breeding, Faculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Omid SofalianDepartment of Agronomy and Plant Breeding, Faculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Behzad SorkhilalehlooSeed and Plant Improvement Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran.
Ali AsghariDepartment of Agronomy and Plant Breeding, Faculty of Agricultural Sciences and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran.
Alireza Pour-AboughadarehSeed and Plant Improvement Institute, Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran. a.poraboghadareh@edu.ikiu.ac.ir.ORCID http://orcid.org/0000-0001-5782-5327

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6 · The paper itself

Abstract

backgroundMaintaining genetic diversity is of the most essential principle for a long-term conservation of plant genetic resources and could play a crucial role in their management. The genus Aegilops is one important member of wheat germplasm, and there are evidences that novel genes of this genus' species can be studied/utilized as ideal sources for the wheat cultivar improvement. The objective of this study was to dissect the genetic diversity and population structure among a set of Iranian Aegilops using two gene-based molecular markers. METHODS AND

resultsThis study investigated the level of genetic diversity among 157 Aegilops accessions consisting of Ae. tauschii Coss. (DD genome), Ae. crassa Boiss. (DDMM genome), and Ae. cylindrica Host. (CCDD genome) belonging to NPGBI using two sets of CBDP and SCoT markers. The SCoT and CBDP primers yielded 171 and 174 fragments, out of which 145 (90.23%) and 167 (97.66%) fragments were polymorphic, respectively. The average of polymorphism information content (PIC)/ marker index (MI)/resolving power (Rp) for SCoT and CBDP markers were 0.32/3.59/16.03 and 0.29/3.01/16.26, respectively. Results of AMOVA revealed the genetic variability within species was greater than the variation observed among them (SCoT: 88% vs. 12%; CBDP: 72% vs. 28%; SCoT + CBDP: 80% vs. 20%). Based on the information obtained from both markers, the higher level of genetic diversity was found in Ae. tauschii as compared to other species. The grouping patterns obtained by Neighbor-joining algorithms, principal coordinate analysis (PCoA), and Bayesian-model-based structure were consistent with each other and resulted in grouping all studied accessions according to their genomic constitutions.

conclusionThe results of this study revealed a high level of genetic diversity among Iranian Aegilops germplasm. Moreover, SCoT and CBDP marker systems were efficient in deciphering DNA polymorphism and classification of Aegilops germplasm.

Indexed as

AegilopsBayes TheoremCodon, InitiatorGenetic MarkersGenetic VariationIranOrganophosphorus CompoundsPolymorphism, Genetic2-(2-cresyl)-4H-1-3-2-benzodioxaphosphorin-2-oxideCodon, InitiatorGenetic MarkersOrganophosphorus CompoundsAegilops spp.Bayesian clustering patternFunctional markersMolecular variability

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PMID37219668

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