ArticleNature materials2023
Photo-expansion microscopy enables super-resolution imaging of cells embedded in 3D hydrogels.
Article in Nature materials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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Who cites it
20 citing papers in PubMed, 34 citations in OpenAlex.
- HiExM Enables Scalable Mapping of Organelle Morphology and Spatial Heterogeneity.bioRxiv : the preprint server for biology · 2026Article
- Review of expansion microscopy combined with advanced imaging modalities.Journal of microscopy · 2026Review
- Activating FGFR1 restores Integrin-β1-mediated fibronectin sensing in satellite cells of aged mice.bioRxiv : the preprint server for biology · 2026Article
- From Light to Life: Molecular Mechanisms and Macroscopic Transformations in Photoresponsive Hydrogels.Polymer science & technology (Washington, D.C.) · 2025Review
- Mesenchymal stem cells: A new strategy for the treatment of femoral head necrosis.Regenerative therapy · 2025Review
- Nuclei sense complex tissue shape and direct intestinal stem cell fate.bioRxiv : the preprint server for biology · 2025Article
- Mesoscale volumetric fluorescence imaging at nanoscale resolution by photochemical sectioning.Science (New York, N.Y.) · 2025Article
- Light-based fabrication and 4D customization of hydrogel biomaterials.Nature reviews bioengineering · 2025Article
- Myofibers cultured in viscoelastic hydrogels reveal the effects of integrin-binding and mechanosensing on muscle satellite cells.Acta biomaterialia · 2025Article
- Nascent matrix deposition supports alveolar organoid formation from aggregates in synthetic hydrogels.Stem cell reports · 2025Article
- Photoresponsive Chemistries for User-Directed Hydrogel Network Modulation to Investigate Cell-Matrix Interactions.Accounts of chemical research · 2025Review
- Polymer design of microwell hydrogels influences epithelial-mesenchymal interactions during human bronchosphere formation.Advanced nanobiomed research · 2025Article
- Article
- HiExM: high-throughput expansion microscopy enables scalable super-resolution imaging.bioRxiv : the preprint server for biology · 2024Article
- Expanding boundaries - a cell biologist's guide to expansion microscopy.Journal of cell science · 2024Review
- SpiDe-Sr: blind super-resolution network for precise cell segmentation and clustering in spatial proteomics imaging.Nature communications · 2024Article
- Nascent matrix deposition supports alveolar organoid formation from aggregates in synthetic hydrogels.bioRxiv : the preprint server for biology · 2024Article
- Mesenchymal Stromal Cell Therapy for Thoracic Surgeons: An Update.Journal of personalized medicine · 2023Review
- Polymer-Based Hydrogels Applied in Drug Delivery: An Overview.Gels (Basel, Switzerland) · 2023Review
- In Situ Super-Resolution Imaging of Organoids and Extracellular Matrix Interactions via Phototransfer by Allyl Sulfide Exchange-Expansion Microscopy (PhASE-ExM).Advanced materials (Deerfield Beach, Fla.) · 2022Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Hydrogels are extensively used as tunable, biomimetic three-dimensional cell culture matrices, but optically deep, high-resolution images are often difficult to obtain, limiting nanoscale quantification of cell-matrix interactions and outside-in signalling. Here we present photopolymerized hydrogels for expansion microscopy that enable optical clearance and tunable ×4.6-6.7 homogeneous expansion of not only monolayer cell cultures and tissue sections, but cells embedded within hydrogels. The photopolymerized hydrogels for expansion microscopy formulation relies on a rapid photoinitiated thiol/acrylate mixed-mode polymerization that is not inhibited by oxygen and decouples monomer diffusion from polymerization, which is particularly beneficial when expanding cells embedded within hydrogels. Using this technology, we visualize human mesenchymal stem cells and their interactions with nascently deposited proteins at <120 nm resolution when cultured in proteolytically degradable synthetic polyethylene glycol hydrogels. Results support the notion that focal adhesion maturation requires cellular fibronectin deposition; nuclear deformation precedes cellular spreading; and human mesenchymal stem cells display cell-surface metalloproteinases for matrix remodelling.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.