Evidence map›Paper›PMID 37217653›Full record

ArticleNature structural & molecular biology2023

Intrinsic mesoscale properties of a Polycomb protein underpin heterochromatin fidelity.

Sujin Lee, Stephen Abini-Agbomson, Daniela S Perry, Allen Goodman, Beiduo Rao, Manning Y Huang, Jolene K Diedrich, James J Moresco, John R Yates, Karim-Jean Armache and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature structural & molecular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Article
  2. HISTONE DEACETYLASE-1 is required for epigenome stability inProceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  3. Article
  4. Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 4 institutions in 1 country.

Sujin LeeDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.ORCID 0000-0003-1411-8686
Stephen Abini-AgbomsonDepartment of Biochemistry and Molecular Pharmacology, New York University Grossman School of Medicine, New York, NY, USA.ORCID 0000-0003-0148-6498
Daniela S PerryDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
Allen GoodmanBroad Institute of MIT and Harvard, Cambridge, MA, USA.
Beiduo RaoDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
Manning Y HuangDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA, USA.
Jolene K DiedrichDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA.
James J MorescoDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA.ORCID 0000-0003-1178-1642
John R YatesDepartment of Molecular Medicine, The Scripps Research Institute, La Jolla, CA, USA.ORCID 0000-0001-5267-1672
Karim-Jean ArmacheDepartment of Biochemistry and Molecular Pharmacology, New York University Grossman School of Medicine, New York, NY, USA.ORCID 0000-0002-0890-9513
Hiten D MadhaniDepartment of Biochemistry and Biophysics, University of California, San Francisco, CA, USA. hitenmadhani@gmail.com.ORCID 0000-0001-7400-6657
University of California, San Francisco · USScripps Research Institute · USNew York University · USBroad Institute · US

Funding

Mechanisms of Gene SilencingR01GM071801 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Hiten D Madhani · 2005 to 2026
$7.1M
Illumina NovaSeq 6000 Sequencing SystemS10OD028511 · OD · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI CHOW, ERIC D · 2020 to 2020
$583k
NIGMS NIH HHS R01 GM071801NIH HHS S10 OD028511
6 · The paper itself

Abstract

Little is understood about how the two major types of heterochromatin domains (HP1 and Polycomb) are kept separate. In the yeast Cryptococcus neoformans, the Polycomb-like protein Ccc1 prevents deposition of H3K27me3 at HP1 domains. Here we show that phase separation propensity underpins Ccc1 function. Mutations of the two basic clusters in the intrinsically disordered region or deletion of the coiled-coil dimerization domain alter phase separation behavior of Ccc1 in vitro and have commensurate effects on formation of Ccc1 condensates in vivo, which are enriched for PRC2. Notably, mutations that alter phase separation trigger ectopic H3K27me3 at HP1 domains. Supporting a direct condensate-driven mechanism for fidelity, Ccc1 droplets efficiently concentrate recombinant C. neoformans PRC2 in vitro whereas HP1 droplets do so only weakly. These studies establish a biochemical basis for chromatin regulation in which mesoscale biophysical properties play a key functional role.

Indexed as

Drosophila ProteinsHeterochromatinChromatinHistonesPolycomb-Group ProteinsChromatinDrosophila ProteinsHeterochromatinHistonesPolycomb-Group Proteins

Identifiers

PMID37217653
PMCPMC11935295
OpenAlexW4377245630

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.