Evidence map›Paper›PMID 37216503›Full record

ArticleProceedings of the National Academy of Sciences of the United States of America2023

Starvation sensing by mycobacterial RelA/SpoT homologue through constitutive surveillance of translation.

Yunlong Li, Soneya Majumdar, Ryan Treen, Manjuli R Sharma, Jamie Corro, Howard B Gamper, Swati R Manjari, Jerome Prusa, Nilesh K Banavali, Christina L Stallings and 3 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Proceedings of the National Academy of Sciences of the United States of America, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.1field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 7 citations in OpenAlex.

  1. Ribosome hibernation in zinc-starvedMicrobiology spectrum · 2026
    Article
  2. Article
  3. Article
  4. Exploring metalloproteome remodeling in calprotectin-stressedbioRxiv : the preprint server for biology · 2025
    Article
  5. Article
  6. MnoSR removal inFrontiers in cellular and infection microbiology · 2024
    Article
  7. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 3 institutions in 1 country.

Yunlong LiDivision of Genetics, New York State Department of Health, Wadsworth Center, Albany, NY 12208.
Soneya MajumdarDivision of Translational Medicine, New York State Department of Health, Wadsworth Center, Albany, NY 12237.
Ryan TreenDivision of Genetics, New York State Department of Health, Wadsworth Center, Albany, NY 12208.ORCID 0000-0001-7212-4262
Manjuli R SharmaDivision of Translational Medicine, New York State Department of Health, Wadsworth Center, Albany, NY 12237.ORCID 0000-0003-4821-748X
Jamie CorroDivision of Genetics, New York State Department of Health, Wadsworth Center, Albany, NY 12208.
Howard B GamperDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107.
Swati R ManjariDivision of Translational Medicine, New York State Department of Health, Wadsworth Center, Albany, NY 12237.
Jerome PrusaDepartment of Molecular Microbiology, Washington University in St. Louis School of Medicine, St. Louis, MO 63110.
Nilesh K BanavaliDivision of Translational Medicine, New York State Department of Health, Wadsworth Center, Albany, NY 12237.ORCID 0000-0003-2206-7049
Christina L StallingsDepartment of Molecular Microbiology, Washington University in St. Louis School of Medicine, St. Louis, MO 63110.ORCID 0000-0002-2747-5618
Ya-Ming HouDepartment of Biochemistry and Molecular Biology, Thomas Jefferson University, Philadelphia, PA 19107.
Rajendra K AgrawalDivision of Translational Medicine, New York State Department of Health, Wadsworth Center, Albany, NY 12237.ORCID 0000-0001-9392-0065
Anil K OjhaDivision of Genetics, New York State Department of Health, Wadsworth Center, Albany, NY 12208.ORCID 0000-0001-5940-2643
New York State Department of Health · USThomas Jefferson University · USWashington University in St. Louis · US

Funding

TRD #3: An automated and streamlined pipeline for in-situ molecular microscopyP41GM103310 · NIGMS · SCRIPPS RESEARCH INSTITUTE, THE · PI DE MARCO, ALEX, KIEFT, JEFFREY S · 2012 to 2021
$14.7M
Structural Aspects of Protein Synthesis in the Mammalian MitochondriaR01GM061576 · NIGMS · WADSWORTH CENTER · PI AGRAWAL, RAJENDRA K · 2001 to 2018
$6.4M
tRNA in codon usageR35GM134931 · NIGMS · THOMAS JEFFERSON UNIVERSITY · PI HOU, YA-MING · 2020 to 2025
$5.4M
Alternative Ribosomes & Antibiotic Tolerance in MycobacteriaR01AI132422 · NIAID · WADSWORTH CENTER · PI RAJENDRA K AGRAWAL, Anil Kumar Ojha · 2017 to 2026
$3.8M
Discovery of Gram-negative permeable chemical probes for tRNA methylationR01AI139202 · NIAID · THOMAS JEFFERSON UNIVERSITY · PI HOU, YA-MING · 2019 to 2022
$2.4M
Role of ribosome modulating proteins in conferring Mycobacterium abscessus antibiotic resistanceR01AI155473 · NIAID · WADSWORTH CENTER · PI GHOSH, PALLAVI · 2020 to 2024
$2.4M
Systematic Discovery and Analysis of Small Proteins and Small ORFs in MycobacteriaR01GM139277 · NIGMS · WADSWORTH CENTER · PI DERBYSHIRE, KEITH M, GRAY, TODD · 2020 to 2023
$2.2M
Targeting a New Essential Virulence Mechanism in Drug-Resistant MycobacteriaR33AI111696 · NIAID · WASHINGTON UNIVERSITY · PI STALLINGS, CHRISTINA LEIGH · 2016 to 2018
$1.4M
Targeting a New Essential Virulence Mechanism in Drug-Resistant MycobacteriaR21AI111696 · NIAID · WASHINGTON UNIVERSITY · PI STALLINGS, CHRISTINA LEIGH · 2014 to 2015
$426k
Origins of zinc starvation in Mycobacterium tuberculosis during chronic infectionR21AI163599 · NIAID · WADSWORTH CENTER · PI OJHA, ANIL KUMAR · 2021 to 2022
$425k
NIAID NIH HHS R01 AI132422NIAID NIH HHS R01 AI139202NIAID NIH HHS R01 AI155473NIAID NIH HHS R21 AI111696NIAID NIH HHS R21 AI163599NIAID NIH HHS R33 AI111696NIGMS NIH HHS P41 GM103310NIGMS NIH HHS R01 GM061576NIGMS NIH HHS R01 GM139277NIGMS NIH HHS R35 GM134931
6 · The paper itself

Abstract

The stringent response, which leads to persistence of nutrient-starved mycobacteria, is induced by activation of the RelA/SpoT homolog (Rsh) upon entry of a deacylated-tRNA in a translating ribosome. However, the mechanism by which Rsh identifies such ribosomes in vivo remains unclear. Here, we show that conditions inducing ribosome hibernation result in loss of intracellular Rsh in a Clp protease-dependent manner. This loss is also observed in nonstarved cells using mutations in Rsh that block its interaction with the ribosome, indicating that Rsh association with the ribosome is important for Rsh stability. The cryo-EM structure of the Rsh-bound 70S ribosome in a translation initiation complex reveals unknown interactions between the ACT domain of Rsh and components of the ribosomal L7/L12 stalk base, suggesting that the aminoacylation status of A-site tRNA is surveilled during the first cycle of elongation. Altogether, we propose a surveillance model of Rsh activation that originates from its constitutive interaction with the ribosomes entering the translation cycle.

Indexed as

MycobacteriumRibosomesRNA, TransferRNA, TransferMpyMycobacteriumRelA/SpoTribosome hibernationzinc

Identifiers

PMID37216503
PMCPMC10235957
OpenAlexW4377292794

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.