Evidence map›Paper›PMID 37216424›Full record

ArticleAngewandte Chemie (International ed. in English)2023

Profiling Phosphoproteome Landscape in Circulating Extracellular Vesicles from Microliters of Biofluids through Functionally Tunable Paramagnetic Separation.

Jie Sun, Qing Li, Yajie Ding, Dong Wei, Marco Hadisurya, Zhuojun Luo, Zhongze Gu, Bobin Chen, W Andy Tao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Angewandte Chemie (International ed. in English), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jie SunState Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing, 210096, China.
Qing LiDepartment of Hematology, Huashan Hospital, Shanghai, 200040, China.
Yajie DingState Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing, 210096, China.
Dong WeiState Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing, 210096, China.
Marco HadisuryaDepartment of Biochemistry, Department of Biochemistry, Purdue University, West Lafayette, IN, 47907, USA.
Zhuojun LuoDepartment of Biochemistry, Department of Biochemistry, Purdue University, West Lafayette, IN, 47907, USA.
Zhongze GuState Key Laboratory of Bioelectronics, National Demonstration Center for Experimental Biomedical Engineering Education, Southeast University, Nanjing, 210096, China.
Bobin ChenDepartment of Hematology, Huashan Hospital, Shanghai, 200040, China.
W Andy TaoDepartment of Biochemistry, Department of Biochemistry, Purdue University, West Lafayette, IN, 47907, USA.ORCID 0000-0002-5535-5517

Funding

Dissecting signaling pathways and seeking EV phosphoproteins as novel biomarkers for Alzheimer's DiseaseRF1AG064250 · NIA · PURDUE UNIVERSITY · PI TAO, W. ANDY · 2020 to 2021
$1.7M
NIA NIH HHS RF1 AG064250
6 · The paper itself

Abstract

Many biological processes are regulated through dynamic protein phosphorylation. Monitoring disease-relevant phosphorylation events in circulating biofluids is highly appealing but also technically challenging. We introduce here a functionally tunable material and a strategy, extracellular vesicles to phosphoproteins (EVTOP), which achieves one-pot extracellular vesicles (EVs) isolation, extraction, and digestion of EV proteins, and enrichment of phosphopeptides, with only a trace amount of starting biofluids. EVs are efficiently isolated by magnetic beads functionalized with Ti

Indexed as

Extracellular VesiclesPhosphopeptidesHumansPhosphoproteinsProteomePhosphopeptidesPhosphoproteinsProteomeCerebrospinal FluidExtracellular VesiclesPhosphoproteomicsProtein ModificationsProteomics

Identifiers

PMID37216424
PMCPMC11019431

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.