Evidence map›Paper›PMID 37216034›Full record

ArticleJournal of tissue engineering

Single-cell RNA-sequence analysis of human bone marrow reveals new targets for isolation of skeletal stem cells using spherical nucleic acids.

Elloise Z Matthews, Stuart Lanham, Kate White, Maria-Eleni Kyriazi, Konstantina Alexaki, Afaf H El-Sagheer, Tom Brown, Antonios G Kanaras, Jonathan J West, Ben D MacArthur and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of tissue engineering. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 5 countries.

Elloise Z MatthewsFaculty of Medicine, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Sciences, University of Southampton, Southampton, UK.ORCID https://orcid.org/0000-0002-9601-4583
Stuart LanhamFaculty of Medicine, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Sciences, University of Southampton, Southampton, UK.
Kate WhiteFaculty of Medicine, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Sciences, University of Southampton, Southampton, UK.
Maria-Eleni KyriaziCollege of Engineering and Technology, American University of the Middle East, Kuwait.
Konstantina AlexakiPhysics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, Southampton, UK.ORCID https://orcid.org/0000-0002-8702-2696
Afaf H El-SagheerDepartment of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK.
Tom BrownDepartment of Chemistry, Chemistry Research Laboratory, University of Oxford, Oxford, UK.
Antonios G KanarasPhysics and Astronomy, Faculty of Physical Sciences and Engineering, University of Southampton, Southampton, UK.
Jonathan J WestCancer Sciences, Faculty of Medicine, University of Southampton, Southampton, UK.
Ben D MacArthurFaculty of Medicine, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Sciences, University of Southampton, Southampton, UK.
Patrick S StumpfFaculty of Medicine, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Sciences, University of Southampton, Southampton, UK.
Richard Oc OreffoFaculty of Medicine, Centre for Human Development, Stem Cells and Regeneration, Human Development and Health, Institute of Developmental Sciences, University of Southampton, Southampton, UK.
University of Southampton · GBAmerican University of the Middle East · KWChina Medical University · TWSuez University · EGUniversity of Oxford · GB

Funding

Medical Research Council MC_PC_15078Medical Research Council MR/R015651/1
6 · The paper itself

Abstract

There is a wealth of data indicating human bone marrow contains skeletal stem cells (SSC) with the capacity for osteogenic, chondrogenic and adipogenic differentiation. However, current methods to isolate SSCs are restricted by the lack of a defined marker, limiting understanding of SSC fate, immunophenotype, function and clinical application. The current study applied single-cell RNA-sequencing to profile human adult bone marrow populations from 11 donors and identified novel targets for SSC enrichment. Spherical nucleic acids were used to detect these mRNA targets in SSCs. This methodology was able to rapidly isolate potential SSCs found at a frequency of <1 in 1,000,000 in human bone marrow, with the capacity for tri-lineage differentiation in vitro and ectopic bone formation in vivo. The current studies detail the development of a platform to advance SSC enrichment from human bone marrow, offering an invaluable resource for further SSC characterisation, with significant therapeutic impact therein.

Indexed as

bone regenerationnanoparticlessingle-cell RNA-sequencingskeletal stem cellsStem cells

Identifiers

PMID37216034
PMCPMC10192814
OpenAlexW4377007438

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.