ArticleNanophotonics (Berlin, Germany)2023
High numerical aperture imaging allows chirality measurement in individual collagen fibrils using polarization second harmonic generation microscopy.
Article in Nanophotonics (Berlin, Germany), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 24 citations in OpenAlex.
- Evaluating the applicability of computational super-resolution techniques for harmonic generation microscopy.Scientific reports · 2026Article
- Collagen microarchitecture from polarized light imaging: a biomechanics perspective.Journal of biomedical optics · 2026Review
- Second harmonic generation of myofibrils exhibits a polarization-resolved "gradient" effect.Biomedical optics express · 2025Article
- Impact of paraben on uterine collagen: An integrated and targeted Correlative approach using second harmonic generation microscopy, nanoindentation, and atomic force microscopy.Journal of the mechanical behavior of biomedical materials · 2025Article
- Effect of out of plane orientation on polarization second harmonic generation of single collagen fibrils.Biomedical optics express · 2023Article
- Polarimetric second-harmonic generation microscopy of partially oriented fibers I: Digital modeling.Biophysical journal · 2023Article
- Characterization of pathological stomach tissue using polarization-sensitive second harmonic generation microscopy.Biomedical optics express · 2023Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Second harmonic generation (SHG) microscopy is a commonly used technique to study the organization of collagen within tissues. However, individual collagen fibrils, which have diameters much smaller than the resolution of most optical systems, have not been extensively investigated. Here we probe the structure of individual collagen fibrils using polarization-resolved SHG (PSHG) microscopy and atomic force microscopy. We find that longitudinally polarized light occurring at the edge of a focal volume of a high numerical aperture microscope objective illuminated with linearly polarized light creates a measurable variation in PSHG signal along the axis orthogonal to an individual collagen fibril. By comparing numerical simulations to experimental data, we are able to estimate parameters related to the structure and chirality of the collagen fibril without tilting the sample out of the image plane, or cutting tissue at different angles, enabling chirality measurements on individual nanostructures to be performed in standard PSHG microscopes. The results presented here are expected to lead to a better understanding of PSHG results from both collagen fibrils and collagenous tissues. Further, the technique presented can be applied to other chiral nanoscale structures such as microtubules, nanowires, and nanoribbons.
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