Evidence map›Paper›PMID 37214955›Full record

ArticlebioRxiv : the preprint server for biology2023

Towards a human brain EV atlas: Characteristics of EVs from different brain regions, including small RNA and protein profiles.

Yiyao Huang, Tanina Arab, Ashley E Russell, Emily R Mallick, Rajini Nagaraj, Evan Gizzie, Javier Redding-Ochoa, Juan C Troncoso, Olga Pletnikova, Andrey Turchinovich and 2 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 8 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 2 countries.

Yiyao HuangDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Tanina ArabDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Ashley E RussellDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Emily R MallickDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Rajini NagarajMeso Scale Diagnostics, LLC, Rockville, MD, USA.
Evan GizzieMeso Scale Diagnostics, LLC, Rockville, MD, USA.
Javier Redding-OchoaDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Juan C TroncosoDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Olga PletnikovaDepartment of Pathology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Andrey TurchinovichDivision of Cancer Genome Research, German Cancer Research Center DKFZ, Heidelberg, Germany.
David A RoutenbergMeso Scale Diagnostics, LLC, Rockville, MD, USA.
Kenneth W WitwerDepartment of Molecular and Comparative Pathobiology, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
Johns Hopkins University · USMeso Scale Discovery (United States) · USGerman Cancer Research Center · DEPennsylvania State University · US

Funding

Project 2 MeasurementU19AG033655 · NIA · JOHNS HOPKINS UNIVERSITY · PI Abhay Moghekar · 2014 to 2026
$51.5M
Research Education ComponentP30AG066507 · NIA · JOHNS HOPKINS UNIVERSITY · PI Corinne Pettigrew · 2020 to 2026
$29.3M
Next-generation extracellular vesicle biologics to target central nervous system and peripheral reservoirs of HIVR01AI144997 · NIAID · JOHNS HOPKINS UNIVERSITY · PI RAMRATNAM, BHARAT, WITWER, KENNETH W · 2019 to 2023
$4.0M
Novel Approaches to Capture, Sorting, and Characterization of CNS-Origin Extracellular VesiclesR33MH118164 · NIMH · JOHNS HOPKINS UNIVERSITY · PI MACHAIRAKI, VASILIKI, WITWER, KENNETH W · 2020 to 2022
$2.2M
Novel Separation Methods for exRNA Carriers: Extracellular Vesicles, Lipoprotein Particles, and Protein AggregatesUH3CA241694 · NCI · JOHNS HOPKINS UNIVERSITY · PI WITWER, KENNETH W · 2021 to 2022
$1.1M
Discovering novel players in mechanisms of extracellular vesicle release, cargo loading, and early pathogenesis of late-onset Alzheimer's DiseaseR56AG057430 · NIA · JOHNS HOPKINS UNIVERSITY · PI CHENG, LINZHAO, MACHAIRAKI, VASILIKI · 2017 to 2017
$796k
Novel Approaches to Capture, Sorting, and Characterization of CNS-Origin Extracellular VesiclesR21MH118164 · NIMH · JOHNS HOPKINS UNIVERSITY · PI MACHAIRAKI, VASILIKI, WITWER, KENNETH W · 2018 to 2019
$471k
Surface Signatures of Neuronal and Glial Extracellular VesiclesUH2MH118167 · NIMH · MESO SCALE DIAGNOSTICS, LLC · PI ROUTENBERG, DAVID AARON · 2018 to 2019
$444k
NCI NIH HHS UH3 CA241694NIAID NIH HHS R01 AI144997NIA NIH HHS P30 AG066507NIA NIH HHS R56 AG057430NIA NIH HHS U19 AG033655NIMH NIH HHS R21 MH118164NIMH NIH HHS R33 MH118164NIMH NIH HHS UH2 MH118167
6 · The paper itself

Abstract

Extracellular vesicles (EVs) are released from different cell types in the central nervous system (CNS) and play roles in regulating physiological and pathological functions. Although brain-derived EVs (bdEVs) have been successfully collected from brain tissue, there is not yet a "bdEV atlas" of EVs from different brain regions. To address this gap, we separated EVs from eight anatomical brain regions of a single individual and subsequently characterized them by count, size, morphology, and protein and RNA content. The greatest particle yield was from cerebellum, while the fewest particles were recovered from the orbitofrontal, postcentral gyrus, and thalamus regions. EV surface phenotyping indicated that CD81 and CD9 were more abundant than CD63 for all regions. Cell-enriched surface markers varied between brain regions. For example, putative neuronal markers NCAM, CD271, and NRCAM were more abundant in medulla, cerebellum, and occipital regions, respectively. These findings, while restricted to tissues from a single individual, suggest that additional studies are merited to lend more insight into the links between EV heterogeneity and function in the CNS.

Indexed as

Braincerebellumcorpus callosumectosomesexosomesExtracellular vesicleshippocampusmedullaoccipital gyrusorbitofrontalpostcentral gyrusthalamustissue

Identifiers

PMID37214955
PMCPMC10197569
OpenAlexW4375950224

What OpenQuestion holds

Textmetadata
LicenceCC BY-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.