Evidence map›Paper›PMID 37214939›Full record

ArticlebioRxiv : the preprint server for biology2023

A developmental gradient reveals biosynthetic pathways to eukaryotic toxins in monocot geophytes.

Niraj Mehta, Yifan Meng, Richard Zare, Rina Kamenetsky-Goldstein, Elizabeth Sattely

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 10 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 2 countries.

Niraj MehtaDepartment of Chemistry, Stanford University, Stanford, CA, 94305, USA.
Yifan MengDepartment of Chemistry, Stanford University, Stanford, CA, 94305, USA.
Richard ZareDepartment of Chemistry, Stanford University, Stanford, CA, 94305, USA.
Rina Kamenetsky-GoldsteinInstitute of Plant Sciences, ARO, The Volcani Center, Rishon LeZion, Israel.
Elizabeth SattelyDepartment of Chemical Engineering, Stanford University, Stanford, CA, 94305, USA.
Stanford University · USAgricultural Research Organization · IL

Funding

Discovery and Engineering of Plant Natural Product PathwaysR01GM121527 · NIGMS · STANFORD UNIVERSITY · PI SATTELY, ELIZABETH SUSAN · 2017 to 2025
$2.3M
NIGMS NIH HHS R01 GM121527
6 · The paper itself

Abstract

Numerous eukaryotic toxins that accumulate in geophytic plants are valuable in the clinic, yet their biosynthetic pathways have remained elusive. A lead example is the >150 Amaryllidaceae alkaloids (AmAs) including galantamine, an FDA-approved treatment for Alzheimer's disease. We show that while AmAs accumulate to high levels in many tissues in daffodils, biosynthesis is localized to nascent, growing tissue at the base of leaves. A similar trend is found for the production of steroidal alkaloids (e.g. cyclopamine) in corn lily. This model of active biosynthesis enabled elucidation of a complete set of biosynthetic genes for the production of AmAs. Taken together, our work sheds light on the developmental and enzymatic logic of diverse alkaloid biosynthesis in daffodil. More broadly, it suggests a paradigm for biosynthesis regulation in monocot geophytes where plants are protected from herbivory through active charging of newly formed cells with eukaryotic toxins that persist as aboveground tissue develops.

Identifiers

PMID37214939
PMCPMC10197729
OpenAlexW4376273134

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.