Evidence map›Paper›PMID 37214842›Full record

ArticleResearch square2023

Light-field tomographic fluorescence lifetime imaging microscopy.

Yayao Ma, Luzhe Huang, Chandani Sen, Samuel Burri, Claudio Bruschini, Xilin Yang, Robert B Cameron, Gregory A Fishbein, Brigitte N Gomperts, Aydogan Ozcan and 2 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in Research square, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors.

Yayao MaDepartment of Bioengineering, University of California, Los Angeles, CA, USA.
Luzhe HuangDepartment of Bioengineering, University of California, Los Angeles, CA, USA.
Chandani SenUCLA Children's Discovery and Innovation Institute, Mattel Children's Hospital UCLA, Department of Pediatrics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.
Samuel BurriAdvanced Quantum Architecture Laboratory, Ecole Polytechnique Federale de Lausanne, Neuchatel, Switzerland.
Claudio BruschiniAdvanced Quantum Architecture Laboratory, Ecole Polytechnique Federale de Lausanne, Neuchatel, Switzerland.
Xilin YangDepartment of Bioengineering, University of California, Los Angeles, CA, USA.
Robert B CameronDepartment of Thoracic Surgery, University of California, Los Angeles, CA, USA.
Gregory A FishbeinDepartment of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA.
Brigitte N GompertsUCLA Children's Discovery and Innovation Institute, Mattel Children's Hospital UCLA, Department of Pediatrics, David Geffen School of Medicine, UCLA, Los Angeles, CA, USA.ORCID 0000-0002-1809-723X
Aydogan OzcanDepartment of Bioengineering, University of California, Los Angeles, CA, USA.
Edoardo CharbonAdvanced Quantum Architecture Laboratory, Ecole Polytechnique Federale de Lausanne, Neuchatel, Switzerland.
Liang GaoDepartment of Bioengineering, University of California, Los Angeles, CA, USA.ORCID 0000-0002-4296-5586

Funding

Ultrafast BioimagingR35GM128761 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI Liang Gao · 2018 to 2026
$3.2M
Kilohertz 3D Optical Mapping of Atrial Fibrillation in Beating Zebrafish HeartsR01HL165318 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAO, LIANG, HSIAI, TZUNG K · 2022 to 2025
$2.2M
Kilohertz volumetric imaging of neuronal action potentials in awake behaving miceRF1NS128488 · NINDS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI GAO, LIANG, GOLSHANI, PEYMAN · 2022 to 2022
$1.6M
NHLBI NIH HHS R01 HL165318NIGMS NIH HHS R35 GM128761NINDS NIH HHS RF1 NS128488
6 · The paper itself

Abstract

Fluorescence lifetime imaging microscopy (FLIM) is a powerful imaging technique that enables the visualization of biological samples at the molecular level by measuring the fluorescence decay rate of fluorescent probes. This provides critical information about molecular interactions, environmental changes, and localization within biological systems. However, creating high-resolution lifetime maps using conventional FLIM systems can be challenging, as it often requires extensive scanning that can significantly lengthen acquisition times. This issue is further compounded in three-dimensional (3D) imaging because it demands additional scanning along the depth axis. To tackle this challenge, we developed a novel computational imaging technique called light field tomographic FLIM (LIFT-FLIM). Our approach allows for the acquisition of volumetric fluorescence lifetime images in a highly data-efficient manner, significantly reducing the number of scanning steps required compared to conventional point-scanning or line-scanning FLIM imagers. Moreover, LIFT-FLIM enables the measurement of high-dimensional data using low-dimensional detectors, which are typically low-cost and feature a higher temporal bandwidth. We demonstrated LIFT-FLIM using a linear single-photon avalanche diode array on various biological systems, showcasing unparalleled single-photon detection sensitivity. Additionally, we expanded the functionality of our method to spectral FLIM and demonstrated its application in high-content multiplexed imaging of lung organoids. LIFT-FLIM has the potential to open up new avenues in both basic and translational biomedical research.

Identifiers

PMID37214842
PMCPMC10197779

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.