More than magnetic isolation: Dynabeads as strong Raman reporters towards simultaneous capture and identification of targets.

Jongwan Lee, Marissa McDonald, Nikiwe Mhlanga, Jeon Woong Kang, Rohit Karnik, Loza F Tadesse

Abstract readPreprint

In one paragraph

Article in ArXiv, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jongwan LeeDepartment of Mechanical Engineering, MIT, Cambridge, MA, 02139, United States.ORCID 0000-0001-6736-3904

Marissa McDonaldDepartment of Health Sciences & Technology, MIT, Cambridge, MA, 02139, United States.ORCID 0000-0003-4214-0303

Nikiwe MhlangaDepartment of Mechanical Engineering, MIT, Cambridge, MA, 02139, United States.

Jeon Woong KangLaser Biomedical Research Center, G. R. Harrison Spectroscopy Laboratory, MIT, Cambridge, MA, 02139, United States.

Rohit KarnikDepartment of Mechanical Engineering, MIT, Cambridge, MA, 02139, United States.ORCID 0000-0003-0588-9286

Loza F TadesseDepartment of Mechanical Engineering, MIT, Cambridge, MA, 02139, United States.ORCID 0000-0003-2040-8145

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Dynabeads are superparamagnetic particles used for immunomagnetic purification of cells and biomolecules. Post-capture, however, target identification relies on tedious culturing, fluorescence staining and/or target amplification. Raman spectroscopy presents a rapid detection alternative, but current implementations target cells themselves with weak Raman signals. We present antibody-coated Dynabeads as strong Raman reporter labels whose effect can be considered a Raman parallel of immunofluorescent probes. Recent developments in techniques for separating target-bound Dynabeads from unbound Dynabeads makes such an implementation feasible with high specificity. We deploy Dynabeads anti

Indexed as

foodborne illnessimmunomagnetic DynabeadsRaman reportersSalmonella enterica

Identifiers

PMID37214136

PMCPMC10197730

What OpenQuestion holds

Textmetadata

LicenceCC BY

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.