Evidence map›Paper›PMID 37212994›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Polycomb Bodies Detection in Murine Fibromuscular Stroma from Skin, Skeletal Muscles, and Aortic Tissues.

Valentina Rosti, Francesca Gorini, Philina Santarelli, Maria Lucia Sarnicola, Silvia Magnani, Chiara Lanzuolo

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
2.5field-weighted citation impact, top 11% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Valentina Rosti *Institute of Biomedical Technologies, National Research Council, Milan, Italy.
Francesca Gorini *Istituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM, Milan, Italy.
Philina SantarelliIstituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM, Milan, Italy.
Maria Lucia SarnicolaIstituto Nazionale di Genetica Molecolare "Romeo ed Enrica Invernizzi", INGM, Milan, Italy.
Silvia MagnaniCharles River Laboratories Italia S.r.l., Milan, Italy.
Chiara Lanzuolo *Institute of Biomedical Technologies, National Research Council, Milan, Italy. chiara.lanzuolo@cnr.it.
Istituto Nazionale Genetica Molecolare · ITInstitute of Biomedical Technologies · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The regulation of chromatin structure depends on a dynamic, multiple mechanisms that modulate gene expression and constitute the epigenome. The Polycomb group (PcG) of proteins are epigenetic factors involved in the transcriptional repression. Among their multilevel, chromatin-associated functions, PcG proteins mediate the establishment and maintenance of higher-order structures at target genes, allowing the transmission of transcriptional programs throughout the cell cycle.In the nucleus, PcG proteins localize close to the pericentric heterochromatin forming microscopically foci, called Polycomb bodies. Here, to visualize the tissue-specific PcG distribution in the aorta, dorsal skin and hindlimb muscles, we combine a fluorescence-activated cell sorter (FACS)-based method with an immunofluorescence staining.

Indexed as

ChromatinDrosophila ProteinsAnimalsCell NucleusHeterochromatinMiceMuscle, SkeletalPolycomb-Group ProteinsChromatinDrosophila ProteinsHeterochromatinPolycomb-Group ProteinsFresh tissue digestionImmunofluorescenceMurine fibroblast characterizationPolycomb foci

Identifiers

PMID37212994
OpenAlexW4377220340

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.