ArticleMicrobiology spectrum2023
The Effect of Inoculum Size on Antimicrobial Susceptibility Testing of Mycobacterium tuberculosis.
Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Effect of Increased Inoculum Size on Drug Susceptibility of Mycobacterium tuberculosis: Challenges in Reliable Drug Resistance Detection.MicrobiologyOpen · 2026Article
- A novel serum-based medium for drug susceptibility testing of Mycobacterium tuberculosis.BMC microbiology · 2026Article
- Validation of Automated Bacterial Suspension Preparation by ColibriAntibiotics (Basel, Switzerland) · 2025Article
- Phytogenic Silver Nanoparticles Derived fromBioengineering (Basel, Switzerland) · 2025Article
- Synthesis of magnetized ZIF-8 and AgRSC advances · 2025Article
- Role of microplastics in the survival and antimicrobial susceptibility ofFrontiers in microbiology · 2025Article
- A simplified pyrazinamidase test forJournal of clinical microbiology · 2024Article
- Investigation of Antibiotic Resistance ofMicroorganisms · 2024Article
- Comparison of Plaque Accumulation Between Titanium and PEEK Healing Abutments.Journal of functional biomaterials · 2024Article
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Authors and funding
4 authors.
Funding
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Abstract
Phenotypic drug susceptibility testing (DST) requires a standardized amount of inoculum to produce reproducible susceptibility results. The most critical step in the application of DST in Mycobacterium tuberculosis isolates is the preparation of the bacterial inoculum. In this study, the effect of bacterial inoculum prepared in various McFarland turbidities on primary antituberculosis drug susceptibility of M. tuberculosis strains was investigated. Five standard ATCC strains (ATCC 27294 [H37Rv], ATCC 35822 [izoniazid-resistant], ATCC 35838 [rifampicin-resistant], ATCC 35820 [streptomycin-resistant], ATCC 35837 [ethambutol-resistant]) were tested. Inoculums of McFarland standard of 0.5, 1, 2, 3, and 1:100 dilutions of 1 McFarland standard of each strain were used. The effect of inoculum size on DST results was determined by the proportion method in Lowenstein-Jensen (LJ) medium and nitrate reductase assay (NRA) in the LJ medium. In both test methods, the increase in inoculum size did not affect the DST results of the strains. On the contrary, DST results were obtained more rapidly as a result of the use of dense inoculum. DST results obtained in all McFarland turbidities were found to be 100% compatible with the recommended amount of inoculum, 1:100 dilution of 1 McFarland standard (inoculum size of gold standard method). In conclusion, the use of a high amount of inoculum did not change the drug susceptibility profile of tuberculosis bacilli. Minimizing manipulations during the inoculum preparation phase of susceptibility testing, this outcome will decrease the need for equipment and make the test application easier, particularly in developing countries.
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