Evidence map›Paper›PMID 37211640›Full record

ArticleThe journal of trauma and acute care surgery2023

Early lyophilized cryoprecipitate enhances the ADAMTS13/VWF ratio to reduce systemic endotheliopathy and lessen lung injury in a mouse multiple-trauma hemorrhage model.

Ahmad Zeineddin, Feng Wu, Jing-Fei Dong, Roumen Vesselinov, Matthew D Neal, Laurence Corash, Shibani Pati, Rosemary A Kozar

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The journal of trauma and acute care surgery, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.7field-weighted citation impact, top 17% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Ahmad ZeineddinFrom the Shock Trauma Center and the University of Maryland School of Medicine (A.Z., F.W., R.A.K.), Baltimore, Maryland; Bloodworks Research Institute and Hematology Division, Department of Medicine (J.-F.D.), University of Washington School of Medicine, Seattle, Washington; Shock Trauma Anesthesiology Research (STAR) Center and Department of Epidemiology (R.V.), University of Maryland School of Medicine, Baltimore, Maryland; Trauma and Transfusion Medicine Research Center, Department of Surgery (M.D.N.), University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania; Cerus Corporation (L.C.), Concord, California; and Department of Laboratory Medicine (S.P.), University of California San Francisco, San Francisco, California.
Feng Wu
Jing-Fei Dong
Roumen Vesselinov
Matthew D Neal
Laurence Corash
Shibani Pati
Rosemary A Kozar
University of Maryland, Baltimore · USBloodworks Northwest · USCerus (United States) · USUniversity of California, San Francisco · USUniversity of Pittsburgh · US

Funding

Mechanistic Elucidation and Targeted Therapy of Platelet Dysfunction After TraumaR35GM119526 · NIGMS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI NEAL, MATTHEW D · 2016 to 2025
$3.9M
Trauma and Shock-Induced Microvascular Dysregulation and CoagulopathyR01GM140983 · NIGMS · UNIVERSITY OF MARYLAND BALTIMORE · PI DONG, JING-FEI, KOZAR, ROSEMARY A · 2022 to 2025
$2.4M
NIGMS NIH HHS R01 GM140983NIGMS NIH HHS R35 GM119526
6 · The paper itself

Abstract

backgroundRecent studies in severely injured patients suggest an important role of von Willebrand Factor (VWF) and ADAMTS13 in the endotheliopathy of trauma (EoT). We hypothesized that the early use of cryoprecipitate would be effective as an endothelial protector by supplementing physiologic VWF and ADAMTS13 to reverse the EoT. We tested a pathogen-reduced lyophilized cryoprecipitate (LPRC) that could expedite the early administration of cryoprecipitate in the battlefield.

methodsA mouse multiple-trauma model with uncontrolled hemorrhage (UCH) from liver injury was utilized followed by hypotensive resuscitation (mean arterial pressure, 55-60) × 3 hours with lactated Ringer's (LR), fresh frozen plasma (FFP), conventional pathogen-reduced cryoprecipitate (CC), and LPRC. Blood was collected for measurement of syndecan-1, VWF, and ADAMTS13 by ELISA. Lungs were stained for histopathologic injury and syndecan-1 and bronchial alveolar lavage (BAL) fluid harvested for protein as an indicator of permeability. Statistical analysis was by ANOVA followed by Bonferroni correction.

resultsFollowing multiple trauma and UCH, blood loss was similar across groups. Mean volume of resuscitation was higher in the LR group compared with the other resuscitation groups. Lung histopathologic injury, syndecan-1 immunostaining and BAL protein were higher with LR compared with resuscitation with FFP and CC, while LPRC further reduced BAL compared with FFP and CC. The ADAMTS13/VWF ratio was significantly lower in LR but improved with FFP and CC, comparable to shams while LPRC further increased this ratio.

conclusionThe protective effects of CC and LPRC were comparable to FFP in ameliorating the EoT in our murine multiple trauma and UCH model. Lyophilized cryoprecipitate may also provide additional benefit by enhancing the ADAMTS13/VWF ratio. These data provide evidence of the safety and efficacy of LPRC and warrants further investigation for its potential application in military settings once approved for human administration.

Indexed as

Lung InjuryMultiple TraumaADAMTS13 ProteinAnimalsHemorrhageHumansMiceSyndecan-1von Willebrand FactorADAMTS13 ProteinADAMTS13 protein, humanADAMTS13 protein, mouseSyndecan-1von Willebrand Factor

Identifiers

PMID37211640
PMCPMC10389395
OpenAlexW4377221224

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.