ArticleNature communications2023
Engineered symbiotic bacteria interfering Nosema redox system inhibit microsporidia parasitism in honeybees.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers, 1 of them a synthesis that pooled it.
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The trial behind it
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Who cites it
33 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Nosemosis negatively affects honeybee survival: experimental and meta-analytic evidence.Parasitology · 2024Pooled it
- Microsporidia: evolution, infection mechanisms and host impact.Nature reviews. Microbiology · 2026Review
- The Caenorhabditis elegans microbiome impacts microsporidia infection through nutrient limitation and spore inactivation.Nature communications · 2026Article
- Disruption of Antioxidant Defense Systems in Honey Bees and Wild Bees Under Environmental Xenobiotic Pressure.Antioxidants (Basel, Switzerland) · 2026Review
- nce-miR-12220 is a vital regulator for microsporidian to infect honeybee via positive modulation of ATP-A and γ-tubulin genes.PLoS pathogens · 2026Article
- Review
- Insight into the Prospects of RNA Interference for Honey Bee Pathogens and Parasite Control.Insects · 2026Review
- Worms About Town: a citizen science project discovers microsporidian parasites of nematodes through environmental sampling.Biology open · 2026Article
- Serpin 4/5 ofMicroorganisms · 2026Article
- Review
- The honeybee gut microbiome: a novel multidimensional model of antimicrobial resistance transmission and immune homeostasis from environmental interactions to health regulation.FEMS microbiology reviews · 2026Review
- iSymBase: an integrative functional-genomic platform for ecological exploration of insect symbionts.ISME communications · 2026Article
- Comparative Evaluation of qPCR and Digital PCR for the Detection ofVeterinary sciences · 2025Article
- GC Content and Thermal Stability of Double-Stranded RNA: Fragments of MicrosporidiaInternational journal of molecular sciences · 2025Article
- Exploring Eco-Friendly Microbial Strategies for Nosemosis Control in Honeybee.Microorganisms · 2025Review
- The in vitro potential of non-thermal atmospheric pressure plasma against Nosema ceranae infection in honeybees (Apis mellifera).Scientific reports · 2025Article
- Host Specificity ofInsects · 2025Article
- Chinese sacbrood virus mediates m6A modification to target and suppress the expression of hemolymph maintenance gene AF9, exacerbating bee infections.Journal of virology · 2025Article
- Liposome-based RNAi delivery in honeybee for inhibiting parasiteSynthetic and systems biotechnology · 2024Article
- Thioredoxin System in Insects: Uncovering the Roles of Thioredoxins and Thioredoxin Reductase beyond the Antioxidant Defences.Insects · 2024Review
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nosema ceranae is an intracellular parasite invading the midgut of honeybees, which causes serious nosemosis implicated in honeybee colony losses worldwide. The core gut microbiota is involved in protecting against parasitism, and the genetically engineering of the native gut symbionts provides a novel and efficient way to fight pathogens. Here, using laboratory-generated bees mono-associated with gut members, we find that Snodgrassella alvi inhibit microsporidia proliferation, potentially via the stimulation of host oxidant-mediated immune response. Accordingly, N. ceranae employs the thioredoxin and glutathione systems to defend against oxidative stress and maintain a balanced redox equilibrium, which is essential for the infection process. We knock down the gene expression using nanoparticle-mediated RNA interference, which targets the γ-glutamyl-cysteine synthetase and thioredoxin reductase genes of microsporidia. It significantly reduces the spore load, confirming the importance of the antioxidant mechanism for the intracellular invasion of the N. ceranae parasite. Finally, we genetically modify the symbiotic S. alvi to deliver dsRNA corresponding to the genes involved in the redox system of the microsporidia. The engineered S. alvi induces RNA interference and represses parasite gene expression, thereby inhibits the parasitism significantly. Specifically, N. ceranae is most suppressed by the recombinant strain corresponding to the glutathione synthetase or by a mixture of bacteria expressing variable dsRNA. Our findings extend our previous understanding of the protection of gut symbionts against N. ceranae and provide a symbiont-mediated RNAi system for inhibiting microsporidia infection in honeybees.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.