ArticleCell stem cell2023
Multimodal characterization of murine gastruloid development.
Article in Cell stem cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 77 citations in OpenAlex.
- Charting Morphotoxicity With Complementary Embryo Models.Advanced healthcare materials · 2026Article
- From pixels to patterns: the AI revolution in stem cell-derived models.Nature methods · 2026Review
- Regulation of mRNA poly(A) tail length governs mammalian body plan formation in gastruloids.Nature communications · 2026Article
- Retinoic Acid Receptor γ Is a Ligand-Activated Gatekeeper to Stem Cell Developmental Progression.International journal of molecular sciences · 2026Review
- Classification of indeterminate and off-target cell types within human kidney organoid differentiation.iScience · 2026Article
- A quantitative pipeline for whole-mount deep imaging and analysis of multi-layered organoids across scales.eLife · 2026Article
- Investigation of embryogenesis in a dish: the development and application of gastruloid models from pluripotent stem cells.Cell & bioscience · 2026Review
- R-loops shape the H2A.Z landscape and promote balanced lineage allocation during differentiation.Genome research · 2026Article
- The proteomic landscape and temporal dynamics of human and mouse gastruloid development.Nature cell biology · 2026Article
- Retinoic Acid Receptor γ Activity Plays a Critical Role in Regulating Early Mouse Gastruloid Development.International journal of molecular sciences · 2026Article
- From genes to germ layers: virtual twins of gastruloids.NPJ systems biology and applications · 2026Review
- Optogenetic WNT signaling drives germ layer self-organization in a human gastruloid model.bioRxiv : the preprint server for biology · 2026Article
- Modeling Post-Implantation Mammalian Embryogenesis Using AdvancedInternational journal of molecular sciences · 2026Review
- Extracellular Matrix Microstructures Directly Regulate Glutathione Bioavailability in Human Hepatocytes.Biomacromolecules · 2025Article
- Non-cell-autonomous control of mouse gastruloid development by the ultra-conserved lncRNA T-UCstem1.The EMBO journal · 2025Article
- N2B27 media formulations influence gastruloid development.Development (Cambridge, England) · 2025Article
- Cooperative short- and long-range interactions enable robust symmetry breaking and axis formation.bioRxiv : the preprint server for biology · 2025Article
- Spatially resolved genome-wide joint profiling of epigenome and transcriptome with spatial-ATAC-RNA-seq and spatial-CUT&Tag-RNA-seq.Nature protocols · 2025Review
- A spatiotemporal atlas of mouse gastrulation and early organogenesis to explore axial patterning and project in vitro models onto in vivo space.Cell reports · 2025Article
- Size-dependent temporal decoupling of morphogenesis and transcriptional programs in pseudoembryos.Science advances · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
13 authors at 7 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gastruloids are 3D structures generated from pluripotent stem cells recapitulating fundamental principles of embryonic pattern formation. Using single-cell genomic analysis, we provide a resource mapping cell states and types during gastruloid development and compare them with the in vivo embryo. We developed a high-throughput handling and imaging pipeline to spatially monitor symmetry breaking during gastruloid development and report an early spatial variability in pluripotency determining a binary response to Wnt activation. Although cells in the gastruloid-core revert to pluripotency, peripheral cells become primitive streak-like. These two populations subsequently break radial symmetry and initiate axial elongation. By performing a compound screen, perturbing thousands of gastruloids, we derive a phenotypic landscape and infer networks of genetic interactions. Finally, using a dual Wnt modulation, we improve the formation of anterior structures in the existing gastruloid model. This work provides a resource to understand how gastruloids develop and generate complex patterns in vitro.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.