Evidence map›Paper›PMID 37209273›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2023

Optimization of the first extraction protocol for metabolomic studies of Brucella abortus.

Joane M M Corrêa, Mauro L G de Oliveira, Patrícia G de Souza, Paulo M S Filho, Adriana N de Macedo, Adriana F Faria

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Joane M M CorrêaDepartamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil.ORCID http://orcid.org/0000-0002-7332-3447
Mauro L G de OliveiraInstituto Mineiro de Agropecuária, Contagem, MG, 32140-000, Brazil.ORCID http://orcid.org/0000-0001-6509-4624
Patrícia G de SouzaLaboratório Federal de Defesa Agropecuária em Minas Gerais, Ministério da Agricultura, Pecuária e Abastecimento, Pedro Leopoldo, MG, 33600-000, Brazil.ORCID http://orcid.org/0000-0002-9736-8850
Paulo M S FilhoLaboratório Federal de Defesa Agropecuária em Minas Gerais, Ministério da Agricultura, Pecuária e Abastecimento, Pedro Leopoldo, MG, 33600-000, Brazil.ORCID http://orcid.org/0000-0002-3239-2278
Adriana N de MacedoDepartamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil. adrianamacedo@qui.ufmg.br.ORCID http://orcid.org/0000-0002-4125-3755
Adriana F FariaDepartamento de Química, Instituto de Ciências Exatas, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil. adriana@qui.ufmg.br.ORCID http://orcid.org/0000-0002-7320-4907
Universidade Federal de Minas Gerais · BRMinistry of Agriculture, Livestock, and Food Supply · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Brucellosis is a zoonosis prevalent worldwide and very recurrent in less developed or developing regions. This zoonosis affects livestock, generating high financial losses to producers, in addition to transmitting diseases to humans through meat consumption or handling contaminated products and animals. In this study, five extraction methods for Brucella abortus intracellular metabolites, using different solvent compositions and cell membrane disruption procedures, were evaluated. Derivatized extracts were analyzed by GC-HRMS. Raw data were processed in XCMS Online and the results were evaluated through multivariate statistical analysis using the MetaboAnalyst platform. The identification of the extracted metabolites was performed by the Unknowns software using the NIST 17.L library. The extraction performance of each method was evaluated for thirteen representative metabolites, comprising four different chemical classes. Most of these compounds are reported in the cell membrane composition of Gram-negative bacteria. The method based on extraction with methanol/chloroform/water presented the best performance in the evaluation of the extracted compounds and in the statistical results. Therefore, this method was selected for extracting intracellular metabolites from cultures of Brucella abortus for untargeted metabolomics analysis.

Indexed as

Brucella abortusBrucellosisAnimalsHumansMetabolomicsSolventsZoonosesSolventsBrucella abortusExtraction methodGas chromatographyHigh resolution mass spectrometryIntracellular metabolitesUntargeted metabolomics

Identifiers

PMID37209273
PMCPMC10484873
OpenAlexW4377140686

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.