Evidence map›Paper›PMID 37209206›Full record

ArticleWorld journal of microbiology & biotechnology2023

Overexpression of genes by stress-responsive promoters increases protein secretion in Saccharomyces cerevisiae.

Chufan Xiao, Songlyu Xue, Yuyang Pan, Xiufang Liu, Mingtao Huang

Abstract read
PubMed Publisher
In one paragraph

Article in World journal of microbiology & biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.9field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Tailored UPRE2 variants for dynamic gene regulation in yeast.Proceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Chufan XiaoSchool of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
Songlyu XueSchool of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
Yuyang PanSchool of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
Xiufang LiuSchool of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China.
Mingtao HuangSchool of Food Science and Engineering, South China University of Technology, Guangzhou, 510641, China. huangmt@scut.edu.cn.
South China University of Technology · CN

Funding

Fundamental Research Funds for the Central Universities 2022ZYGXZR102Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing 2022B1212010015National Key Research and Development Program of China 2021YFC2100500Science and Technology Program of Guangzhou 202002030337
6 · The paper itself

Abstract

Recombinant proteins produced by cell factories are now widely used in various fields. Many efforts have been made to improve the secretion capacity of cell factories to meet the increasing demand for recombinant proteins. Recombinant protein production usually causes cell stress in the endoplasmic reticulum (ER). The overexpression of key genes possibly removes limitations in protein secretion. However, inappropriate gene expression may have negative effects. There is a need for dynamic control of genes adapted to cellular status. In this study, we constructed and characterized synthetic promoters that were inducible under ER stress conditions in Saccharomyces cerevisiae. The unfolded protein response element UPRE2, responding to stress with a wide dynamic range, was assembled with various promoter core regions, resulting in UPR-responsive promoters. Synthetic responsive promoters regulated gene expression by responding to stress level, which reflected the cellular status. The engineered strain using synthetic responsive promoters P

Indexed as

Saccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsMetabolic EngineeringSaccharomyces cerevisiae ProteinsDynamic controlEndoplasmic reticulum stressPromoter engineeringRecombinant protein

Identifiers

PMID37209206
OpenAlexW4377138675

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.