Evidence map›Paper›PMID 37208611›Full record

ArticleBMC bioinformatics2023

CircPrime: a web-based platform for design of specific circular RNA primers.

Fedor Sharko, Golam Rbbani, Prabhugouda Siriyappagouder, Joost A M Raeymaekers, Jorge Galindo-Villegas, Artem Nedoluzhko, Jorge M O Fernandes

Open access · goldAbstract read
In one paragraph

Article in BMC bioinformatics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 3 countries.

Fedor Sharko *Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prospect 33/2, 119071, Moscow, Russia.
Golam Rbbani *Faculty of Biosciences and Aquaculture, Nord University, Universitetsalléen 11, PB 1490, 8049, Bodø, Norway.
Prabhugouda SiriyappagouderFaculty of Biosciences and Aquaculture, Nord University, Universitetsalléen 11, PB 1490, 8049, Bodø, Norway.
Joost A M RaeymaekersFaculty of Biosciences and Aquaculture, Nord University, Universitetsalléen 11, PB 1490, 8049, Bodø, Norway.
Jorge Galindo-VillegasFaculty of Biosciences and Aquaculture, Nord University, Universitetsalléen 11, PB 1490, 8049, Bodø, Norway.
Artem NedoluzhkoFaculty of Biosciences and Aquaculture, Nord University, Universitetsalléen 11, PB 1490, 8049, Bodø, Norway. nedoluzhko@gmail.com.ORCID http://orcid.org/0000-0001-7040-0892
Jorge M O FernandesFaculty of Biosciences and Aquaculture, Nord University, Universitetsalléen 11, PB 1490, 8049, Bodø, Norway. jorge.m.fernandes@nord.no.
Nord University · NOEuropean University at Saint Petersburg · RUObayashi (Japan) · JP

Funding

European Research Council 683210
6 · The paper itself

Abstract

backgroundCircular RNAs (circRNAs) are covalently closed-loop RNAs with critical regulatory roles in cells. Tens of thousands of circRNAs have been unveiled due to the recent advances in high throughput RNA sequencing technologies and bioinformatic tools development. At the same time, polymerase chain reaction (PCR) cross-validation for circRNAs predicted by bioinformatic tools remains an essential part of any circRNA study before publication.

resultsHere, we present the CircPrime web-based platform, providing a user-friendly solution for DNA primer design and thermocycling conditions for circRNA identification with routine PCR methods.

conclusionsUser-friendly CircPrime web platform ( http://circprime.elgene.net/ ) works with outputs of the most popular bioinformatic predictors of circRNAs to design specific circular RNA primers. CircPrime works with circRNA coordinates and any reference genome from the National Center for Biotechnology Information database).

Indexed as

RNARNA, CircularComputational BiologyInternetPolymerase Chain ReactionSequence Analysis, RNARNARNA, CircularcircRNAsCircular RNAsPCRPredictionPrimer designqPCRRNA-sequencingRT-PCRValidationWeb platform

Identifiers

PMID37208611
PMCPMC10197314
OpenAlexW4377096953

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.