ArticleScience advances2023
Multiscale stiffness of human emphysematous precision cut lung slices.
Article in Science advances, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- 4D Time-lapse imaging of cell dynamics in human alveolar tissue by label-free higher harmonic generation microscopy.Journal of translational medicine · 2026Article
- A new tunable 3D alveolospheres model from human alveolar epithelial type 2 cells (AEC2) with reduced heterogeneity for studying cigarette smoke extract exposure.Respiratory research · 2026Article
- Human ex-vivo lung mechanics differ under positive- and negative-pressure ventilation.Respiratory research · 2026Article
- The importance of mechanical forces in chronic respiratory diseases.European respiratory review : an official journal of the European Respiratory Society · 2026Review
- Emphysema Phenotype and 1‑Year Readmission Risk After Acute Exacerbation of COPD: A Real-World Study Using Propensity Score-Based Methods.International journal of chronic obstructive pulmonary disease · 2026Article
- Imaging the extracellular matrix structure and remodeling in healthy, fibrotic, and emphysematous human precision-cut lung slices.Respiratory research · 2025Article
- Label-free four-dimensional live imaging of immune-stromal interactions in human alveolar tissue using higher harmonic generation microscopy.The European respiratory journal · 2025Article
- Living human lung slices for ex vivo modelling of lung cancer.JCI insight · 2025Article
- Cryopreservation of human lung tissue for 3D ex vivo analysis.Respiratory research · 2025Review
- Tissue-Informed Biomaterial Innovations Advance Pulmonary Regenerative Engineering.ACS macro letters · 2025Review
- Desmosine: The Rationale for Its Use as a Biomarker of Therapeutic Efficacy in the Treatment of Pulmonary Emphysema.Diagnostics (Basel, Switzerland) · 2025Article
- Precision-Cut Lung Slices: Emerging Tools for Preclinical and Translational Lung Research: An Official American Thoracic Society Workshop Report.American journal of respiratory cell and molecular biology · 2025Article
- The Potential Role of Cigarette Smoke, Elastic Fibers, and Secondary Lung Injury in the Transition of Pulmonary Emphysema to Combined Pulmonary Fibrosis and Emphysema.International journal of molecular sciences · 2024Article
- A 3D Epithelial-Mesenchymal Co-Culture Model of the Airway Wall Using Native Lung Extracellular Matrix.Bioengineering (Basel, Switzerland) · 2024Article
- The Fibrotic Phenotype of Human Precision-Cut Lung Slices Is Maintained after Cryopreservation.Toxics · 2024Article
- Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery.Respiratory research · 2024Review
- Mapping the strain-stiffening behavior of the lung and lung cancer at microscale resolution using the crystal ribcage.Frontiers in network physiology · 2024Article
- Elucidating the interaction between stretch and stiffness using an agent-based spring network model of progressive pulmonary fibrosis.Frontiers in network physiology · 2024Article
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
Emphysema is a debilitating disease that remodels the lung leading to reduced tissue stiffness. Thus, understanding emphysema progression requires assessing lung stiffness at both the tissue and alveolar scales. Here, we introduce an approach to determine multiscale tissue stiffness and apply it to precision-cut lung slices (PCLS). First, we established a framework for measuring stiffness of thin, disk-like samples. We then designed a device to verify this concept and validated its measuring capabilities using known samples. Next, we compared healthy and emphysematous human PCLS and found that the latter was 50% softer. Through computational network modeling, we discovered that this reduced macroscopic tissue stiffness was due to both microscopic septal wall remodeling and structural deterioration. Lastly, through protein expression profiling, we identified a wide spectrum of enzymes that can drive septal wall remodeling, which, together with mechanical forces, lead to rupture and structural deterioration of the emphysematous lung parenchyma.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.