Evidence map›Paper›PMID 37205253›Full record

ReviewMovement disorders clinical practice2023

α-Synuclein Seed Amplification Assays in the Diagnosis of Synucleinopathies Using Cerebrospinal Fluid-A Systematic Review and Meta-Analysis.

Anna Grossauer, Greta Hemicker, Florian Krismer, Marina Peball, Atbin Djamshidian, Werner Poewe, Klaus Seppi, Beatrice Heim

Open access · hybridAbstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Movement disorders clinical practice, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 63 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
63citing papers in PubMed, 2 pooled it
20.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

63 citing papers in PubMed, 2 syntheses or guidelines pooled it, 101 citations in OpenAlex.

  1. Pooled it
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  5. Multiple system atrophy: cure and care.Journal of neural transmission (Vienna, Austria : 1996) · 2026
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  11. Clinical and Imaging Characteristics of Parkinson's Disease with Negative Alpha-Synuclein Seed Amplification Assay.Movement disorders : official journal of the Movement Disorder Society · 2026
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3 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Anna GrossauerDepartment of Neurology Medical University of Innsbruck Innsbruck Austria.ORCID https://orcid.org/0000-0002-8611-8055
Greta HemickerDepartment of Neurology Medical University of Innsbruck Innsbruck Austria.
Florian KrismerDepartment of Neurology Medical University of Innsbruck Innsbruck Austria.ORCID https://orcid.org/0000-0002-4493-5073
Marina PeballDepartment of Neurology Medical University of Innsbruck Innsbruck Austria.ORCID https://orcid.org/0000-0002-0791-7300
Atbin DjamshidianDepartment of Neurology Medical University of Innsbruck Innsbruck Austria.
Werner PoeweDepartment of Neurology Medical University of Innsbruck Innsbruck Austria.
Klaus SeppiDepartment of Neurology Medical University of Innsbruck Innsbruck Austria.ORCID https://orcid.org/0000-0001-6503-1455
Beatrice HeimDepartment of Neurology Medical University of Innsbruck Innsbruck Austria.ORCID https://orcid.org/0000-0002-6749-3044
Innsbruck Medical University · AT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Real-time quaking-induced conversion (RT-QuIC) and protein misfolding cyclic amplification (PMCA) have been developed to detect minute amounts of amyloidogenic proteins via amplification techniques and have been used to detect misfolded α-synuclein (αSyn) aggregates in the cerebrospinal fluid (CSF) and other source materials of patients with Parkinson's Disease and other synucleinopathies. Objectives: The aim of this systematic review and meta-analysis was to evaluate the diagnostic accuracy of αSyn seed amplification assays (αSyn-SAAs), including RT-QuIC and PMCA, using CSF as source material to differentiate synucleinopathies from controls. Methods: The electronic MEDLINE database PubMed was searched for relevant articles published until June 30, 2022. Study quality assessment was performed using the QUADAS-2 toolbox. A random effects bivariate model was exploited for data synthesis. Results: Our systematic review identified 27 eligible studies according to the predefined inclusion criteria, of which 22 were included in the final analysis. Overall, 1855 patients with synucleinopathies and 1378 non-synucleinopathies as control subjects were included in the meta-analysis. The pooled sensitivity and specificity to differentiate synucleinopathies from controls with αSyn-SAA were 0.88 (95% CI, 0.82-0.93) and 0.95 (95% CI, 0.92-0.97), respectively. Evaluating the diagnostic performance of RT-QuIC in a subgroup analysis for the detection of patients with multiple system atrophy the pooled sensitivity decreased to 0.30 (95% CI, 0.11-0.59). Conclusions: While our study clearly demonstrated a high diagnostic performance of RT-QuIC and PMCA for differentiating synucleinopathies with Lewy bodies from controls, results for the diagnosis of multiple system atrophy were less robust.

Indexed as

alpha‐synucleinbiomarkerprotein misfolding cyclic amplification (PMCA)real‐time quaking‐induced conversion (RT‐QuIC)synucleinopathy

Identifiers

PMID37205253
PMCPMC10187020
OpenAlexW4323045388

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.