Evidence map›Paper›PMID 37204729›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Whole Blood Procoagulant Platelet Flow Cytometry Protocol for Heparin-Induced Thrombocytopenia (HIT) and Vaccine-Induced Immune Thrombotic Thrombocytopenia (VITT) Testing.

Christine S M Lee, Matthew C Powell, Vivien M Chen

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
17.0field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 3 institutions in 2 countries.

Christine S M LeeANZAC Research Institute, University of Sydney, Sydney, NSW, Australia.ORCID 0000-0002-3851-9364
Matthew C PowellDiagnostic Pathology Unit, NSW Health Pathology, Concord Hospital, Concord, NSW, Australia.ORCID 0000-0001-8621-6328
Vivien M ChenANZAC Research Institute, University of Sydney, Sydney, NSW, Australia. Vivien.chen@sydney.edu.au.ORCID 0000-0001-5119-7470
Concord Hospital · USConcord Repatriation General Hospital · AUThe University of Sydney · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heparin-induced thrombocytopenia (HIT) is a well-characterized, iatrogenic complication of heparin anticoagulation with significant morbidity. In contrast, vaccine-induced immune thrombotic thrombocytopenia (VITT) is a recently recognized severe prothrombotic complication of adenoviral vaccines, including the ChAdOx1 nCoV-19 (Vaxzevria, AstraZeneca) and Ad26.COV2.S (Janssen, Johnson & Johnson) vaccines against COVID-19. The diagnosis of HIT and VITT involve laboratory testing for antiplatelet antibodies by immunoassays followed by confirmation by functional assays to detect platelet-activating antibodies. Functional assays are critical to detect pathological antibodies due to the varying sensitivity and specificity of immunoassays. This chapter presents a protocol for a novel whole blood flow cytometry-based assay to detect procoagulant platelets in healthy donor blood in response to plasma from patients suspected of HIT or VITT. A method to identify suitable healthy donors for HIT and VITT testing is also described.

Indexed as

COVID-19ThrombocytopeniaThrombosisVaccinesAd26COVS1AntibodiesBlood PlateletsChAdOx1 nCoV-19COVID-19 VaccinesFlow CytometryHumansPlatelet Factor 4Ad26COVS1AntibodiesChAdOx1 nCoV-19COVID-19 VaccinesPlatelet Factor 4VaccinesFlow cytometryHeparin-induced thrombocytopeniaHITPF4 antibodiesProcoagulant plateletVaccine-induced immune thrombotic thrombocytopeniaVITTWhole blood

Identifiers

PMID37204729
OpenAlexW4377093503

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.