ArticleThe Plant cell2023
Plant polygalacturonase structures specify enzyme dynamics and processivities to fine-tune cell wall pectins.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 40 citations in OpenAlex.
- Close contact: role of the extracellular matrix in cell adhesion and separation during plant development and reproduction.Plant reproduction · 2026Review
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- DArTseq molecular markers associated with water stress tolerance and size reduction in citrandarins rootstocks.Frontiers in plant science · 2026Article
- Symbiosome functionality in Medicago truncatula nodules requires continuous clearing of pectins from the symbiosome space.Nature communications · 2025Article
- Hunting for the genetic loci underlying root lodging resistance by BSR-seq and QTL mapping in maize.BMC plant biology · 2025Article
- The role of plant cell walls in pest resistance: current insights and future perspectives.Journal of experimental botany · 2025Review
- Circular cell clusters and calcium oxalate crystals: critical players in Solanaceae anther dehiscence.Planta · 2025Review
- PbrBGAL6 promotes pollen tube growth by influencing apical pectin level in Pyrus bretschneideri.BMC genomics · 2025Article
- Potential of Bio-Sourced Oligogalacturonides in Crop Protection.Molecules (Basel, Switzerland) · 2025Review
- Jasmonic Acid (JA) Signaling Pathway in Rice Defense Against Chilo suppressalis Infestation.Rice (New York, N.Y.) · 2025Review
- Structural glycobiology - from enzymes to organelles.Biochemical Society transactions · 2025Review
- Revisiting interactions between polygalacturonases and polygalacturonase-inhibiting proteins and their effects on plant health: a review.Frontiers in plant science · 2025Review
- Article
- Structure and growth of plant cell walls.Nature reviews. Molecular cell biology · 2024Review
- Phenotypic Investigation and RNA-seq ofInternational journal of molecular sciences · 2024Article
- Comparative evaluation of physiological and molecular responses of blackcurrant varieties to powdery mildew infection.Frontiers in plant science · 2024Article
- Enzymes in 3D: Synthesis, remodelling, and hydrolysis of cell wall (1,3;1,4)-β-glucans.Plant physiology · 2023Article
- In muro matters: Structural differences of polygalacturonases define their specific roles in plant development.The Plant cell · 2023Article
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22 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Polygalacturonases (PGs) fine-tune pectins to modulate cell wall chemistry and mechanics, impacting plant development. The large number of PGs encoded in plant genomes leads to questions on the diversity and specificity of distinct isozymes. Herein, we report the crystal structures of 2 Arabidopsis thaliana PGs, POLYGALACTURONASE LATERAL ROOT (PGLR), and ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE2 (ADPG2), which are coexpressed during root development. We first determined the amino acid variations and steric clashes that explain the absence of inhibition of the plant PGs by endogenous PG-inhibiting proteins (PGIPs). Although their beta helix folds are highly similar, PGLR and ADPG2 subsites in the substrate binding groove are occupied by divergent amino acids. By combining molecular dynamic simulations, analysis of enzyme kinetics, and hydrolysis products, we showed that these structural differences translated into distinct enzyme-substrate dynamics and enzyme processivities: ADPG2 showed greater substrate fluctuations with hydrolysis products, oligogalacturonides (OGs), with a degree of polymerization (DP) of ≤4, while the DP of OGs generated by PGLR was between 5 and 9. Using the Arabidopsis root as a developmental model, exogenous application of purified enzymes showed that the highly processive ADPG2 had major effects on both root cell elongation and cell adhesion. This work highlights the importance of PG processivity on pectin degradation regulating plant development.
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