ArticleVeterinary medicine and science2023
The influence of L-proline and fulvic acid on oxidative stress and semen quality of buffalo bull semen following cryopreservation.
Article in Veterinary medicine and science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 50 citations in OpenAlex.
- Nicotinic and Folic Acid Supplementation Improves Cryopreserved Ram Sperm Quality, Potentially Through Modulation ofInternational journal of molecular sciences · 2026Article
- Dose-dependent protective effects of p-coumaric acid on testicular ischemia/reperfusion injury in a mouse model of testicular torsion.Biochemistry and biophysics reports · 2026Article
- Thiram inhibits human sperm motility and capacitation by increasing mitochondrial OTranslational andrology and urology · 2026Article
- Article
- Synergistic amelioration of heat stress-induced testicular damage and impaired fertility by sesame seed oil and α-lipoic acid in male rats.Basic and clinical andrology · 2026Article
- Dictyota dichotoma extract as a potential nutraceutical for male fertility: insights into semen quality, testicular histology, immunomodulation, and anti-inflammatory markers in rabbit bucks.BMC veterinary research · 2026Article
- Naringin attenuates testicular ischemia/reperfusion injury in a mouse torsion/detorsion model via intraperitoneal administration.Pediatric surgery international · 2026Article
- Moringa oleifera Lam. As a Herbal Antioxidant-Enriched Semen Extender in Cryopreservation: A Critical Review of Its Protective Potential.Veterinary medicine and science · 2026Review
- Article
- Canthaxanthin enhances the quality of cryopreserved goat spermatozoa.Veterinary research forum : an international quarterly journal · 2026Article
- Efficacy of zinc oxide nanoparticles in chemical castration of male Wistar rats.Scientific reports · 2025Article
- Intratesticular copper oxide nanoparticles induce dose-dependent chemical castration in male mice.Scientific reports · 2025Article
- Effect of gallic acid in mitigating hepatorenal injuries induced by gentamicin administration in male Wistar rats.Naunyn-Schmiedeberg's archives of pharmacology · 2025Article
- Molecular weight-based profiling of sperm proteins and their association with semen quality in Kokok Balenggek roosters.Journal of advanced veterinary and animal research · 2025Article
- Oral niacin mitigates heat-induced reproductive impairments in male mice.Scientific reports · 2025Article
- Impact of Cryopreservation Extenders on Epigenetic Changes in Bull Sperm: H19 and MEG3 Methylation.Veterinary medicine and science · 2025Article
- L-Proline supplementation preserves sperm function and chromatin integrity in asthenozoospermic patients during cryopreservation.Basic and clinical andrology · 2025Article
- Comparison of Tree-Based Machine Learning Algorithms for Classification of Livestock Breeds Based On Post-Thaw Spermatological Parameters.Veterinary medicine and science · 2025Article
- β-Glucan and resveratrol mitigate lead induced reproductive toxicity in male mice.Scientific reports · 2025Article
- The Effects of Limonin, Myo-Inositol, and L-Proline on the Cryopreservation of Debao Boar Semen.Animals : an open access journal from MDPI · 2025Article
Corrections and comments
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Authors and funding
11 authors at 7 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThis study investigates the effects of cryopreservation and supplementation of Azeri water buffalo's semen with proline (Lp) and fulvic acid (FA).
objectivesTherefore, this study aimed to assess motility parameters, sperm viability, oxidative stress parameters, and DNA damage to detect the optimum concentrations of Lp and FA for buffalo semen cryopreservation.
methodsThirty semen samples of three buffalo bulls were diluted in Tris-egg yolk extender and divided into 12 equal groups including control (C), Lp-10, Lp-20, Lp-40, Lp-60, Lp-80 (containing 10, 20, 40, 60, 80 mM L-proline, respectively), FA-0.2, FA-0.5, FA-0.8, FA-1.1, FA-1.4 and FA-1.7 (containing 0.2%, 0.5%, 0.8%, 1.1%, 1.4% and 1.7% fulvic acid, respectively).
resultsThe velocity parameters, TM and PM were improved by FA-1.7, FA-1.4, Lp-40 and Lp-60 groups compared to the C group but no significant difference was found regarding the amplitude of lateral head displacement and straightness compared to the control groups. The percentage of sperm viability and PMF were increased by FA-1.7, FA-1.4, FA-1.1, Lp-40 and Lp-60 groups compared to C group, while in terms of sperm DNA damage FA-1.7, FA-1.4, FA-1.1, Lp-10, Lp-20, Lp-40 and Lp-60 groups showed better results compared to C group. The results also showed that FA-1.7, FA-1.4, FA-1.1, Lp-20, Lp-40 and Lp-60 groups could improve TAC, SOD, GSH and decrease MDA levels. Also, FA-1.7, FA-1.4, Lp-20 and Lp-40 groups could improve GPx levels but just FA-1.7, and Lp-40 groups could improve CAT levels compared to C group.
conclusionsThus, it can be concluded that L-proline and fulvic acid supplementations can improve the quality parameters of post-thawed buffalo bull semen.
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