Evidence map›Paper›PMID 37196130›Full record

ArticleAging and disease2023

Optogenetic Activation of Astrocytes Reduces Blood-Brain Barrier Disruption via IL-10 In Stroke.

Qian Suo, Lidong Deng, Tingting Chen, Shengju Wu, Lin Qi, Ze Liu, Tingting He, Heng-Li Tian, Wanlu Li, Yaohui Tang and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Aging and disease, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
2.8field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 19 citations in OpenAlex.

  1. Review
  2. Review
  3. Hemodynamic and Electrophysiological Progression of the Rose Bengal Photothrombotic Stroke Model in Mice.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2026
    Article
  4. Review
  5. Article
  6. Review
  7. Article
  8. Review
  9. Article
  10. Review
  11. Review
  12. Review
  13. Article
  14. Article
  15. Hemorrhagic stroke-induced subtype of inflammatory reactive astrocytes disrupts blood-brain barrier.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2024
    Article
  16. Article
  17. Optogenetics in Alzheimer's Disease: Focus on Astrocytes.Antioxidants (Basel, Switzerland) · 2023
    Review
  18. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Qian SuoShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Lidong DengShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Tingting ChenShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Shengju WuShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Lin QiShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Ze LiuShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Tingting HeDepartment of Neurology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.
Heng-Li TianDepartment of Neurosurgery, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai, China.
Wanlu LiShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Yaohui TangShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Guo-Yuan YangShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Zhijun ZhangShanghai Jiao Tong Affiliated Sixth People's Hospital, and School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Shanghai Jiao Tong University · CNShanghai Tenth People's Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Optogenetics has been used to regulate astrocyte activity and modulate neuronal function after brain injury. Activated astrocytes regulate blood-brain barrier functions and are thereby involved in brain repair. However, the effect and molecular mechanism of optogenetic-activated astrocytes on the change in barrier function in ischemic stroke remain obscure. In this study, adult male GFAP-ChR2-EYFP transgenic Sprague-Dawley rats were stimulated by optogenetics at 24, 36, 48, and 60 h after photothrombotic stroke to activate ipsilateral cortical astrocytes. The effects of activated astrocytes on barrier integrity and the underlying mechanisms were explored using immunostaining, western blotting, RT-qPCR, and shRNA interference. Neurobehavioral tests were performed to evaluate therapeutic efficacy. The results demonstrated that IgG leakage, gap formation of tight junction proteins, and matrix metallopeptidase 2 expression were reduced after optogenetic activation of astrocytes (p<0.05). Moreover, photo-stimulation of astrocytes protected neurons against apoptosis and improved neurobehavioral outcomes in stroke rats compared to controls (p<0.05). Notably, interleukin-10 expression in optogenetic-activated astrocytes significantly increased after ischemic stroke in rats. Inhibition of interleukin-10 in astrocytes compromised the protective effects of optogenetic-activated astrocytes (p<0.05). We found for the first time that interleukin-10 derived from optogenetic-activated astrocytes protected blood-brain barrier integrity by decreasing the activity of matrix metallopeptidase 2 and attenuated neuronal apoptosis, which provided a novel therapeutic approach and target in the acute stage of ischemic stroke.

Identifiers

PMID37196130
PMCPMC10529757
OpenAlexW4360595365

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.