ArticleThe Plant cell2023
A phase-separated CO2-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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15 citing papers in PubMed, 28 citations in OpenAlex.
- Best practices for biotin ligase-based proximity labeling proteomics in plant systems.The Plant cell · 2026Review
- EPOS1 is required for normal pyrenoid starch sheath formation and efficiency of the COThe New phytologist · 2026Article
- Application of Proximity-Labeling Techniques in Plants: A Review of Successful Cases.International journal of molecular sciences · 2026Review
- Partitioning of Rubisco activase into the pyrenoidal Rubisco condensate is mediated by a functional protein-protein interaction.Nature communications · 2026Article
- The study of plasmodesmal biology using proximity labeling technologies.Journal of experimental botany · 2026Review
- Proteomic analysis of the pyrenoid-traversing membranes of Chlamydomonas reinhardtii reveals novel components.The New phytologist · 2026Article
- Proteomic analysis of the pyrenoid-traversing membranes ofbioRxiv : the preprint server for biology · 2025Article
- The thylakoid- and pyrenoid-localized phosphate transporter PHT4-9 is essential for photosynthesis in Chlamydomonas.Plant physiology · 2025Article
- The activation ofPlant direct · 2024Article
- Pyrenoid proteomics reveals independent evolution of the COProceedings of the National Academy of Sciences of the United States of America · 2024Article
- TurboID reveals the proxiomes of Chlamydomonas proteins involved in thylakoid biogenesis and stress response.Plant physiology · 2023Article
- TurboID reveals novel pyrenoid proteins in algae.The Plant cell · 2023Article
- The pyrenoid: the eukaryotic CO2-concentrating organelle.The Plant cell · 2023Article
- Focus on biomolecular condensates.The Plant cell · 2023Article
- Chloroplast protein complexes identified by TurboID in Chlamydomonas reinhardtii.Plant physiology · 2023Article
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5 authors at 1 institution in 1 country.
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Abstract
Phase separation underpins many biologically important cellular events such as RNA metabolism, signaling, and CO2 fixation. However, determining the composition of a phase-separated organelle is often challenging due to its sensitivity to environmental conditions, which limits the application of traditional proteomic techniques like organellar purification or affinity purification mass spectrometry to understand their composition. In Chlamydomonas reinhardtii, Rubisco is condensed into a crucial phase-separated organelle called the pyrenoid that improves photosynthetic performance by supplying Rubisco with elevated concentrations of CO2. Here, we developed a TurboID-based proximity labeling technique in which proximal proteins in Chlamydomonas chloroplasts are labeled by biotin radicals generated from the TurboID-tagged protein. By fusing 2 core pyrenoid components with the TurboID tag, we generated a high-confidence pyrenoid proxiome that contains most known pyrenoid proteins, in addition to new pyrenoid candidates. Fluorescence protein tagging of 7 previously uncharacterized TurboID-identified proteins showed that 6 localized to a range of subpyrenoid regions. The resulting proxiome also suggests new secondary functions for the pyrenoid in RNA-associated processes and redox-sensitive iron-sulfur cluster metabolism. This developed pipeline can be used to investigate a broad range of biological processes in Chlamydomonas, especially at a temporally resolved suborganellar resolution.
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