ArticlePlant biotechnology journal2023
The RUBY reporter enables efficient haploid identification in maize and tomato.
Article in Plant biotechnology journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- RUBY expression alters development and metabolism in Arabidopsis.Plant molecular biology · 2026Article
- A RUBY-Based Visual Hairy Root Transformation and CRISPR-Plants (Basel, Switzerland) · 2026Article
- Development of an Optimized in Planta Transformation System in Sugarcane and Its Application onPlants (Basel, Switzerland) · 2026Article
- Expanding agroinfiltration host range with broad-spectrum effectors monitored by autoluminescent reporter.Horticulture research · 2026Article
- Rooting Conifer Genetic Research: An Accessible and Efficient Transformation System.Plant biotechnology journal · 2026Article
- Visual marker-assisted gene editing ofaBIOTECH · 2026Article
- Stepwise construction of the path to doubled haploid breeding in sorghum.Science advances · 2026Article
- Betacyanin biofortification in wheat via synthetic biology for nutritional enhancement.Plant physiology · 2026Article
- Article
- Rapid Genetic Stabilisation of Transgenic and Edited Wheat Through Transformation of Immature Haploid Embryos.Plant biotechnology journal · 2026Article
- Recent advances in generation of doubled haploid plants for genetic improvement in solanaceous vegetable crops.Planta · 2026Review
- Improved efficiency of genetic transformation for the development of elite germplasm using CRISPR/Cas12i3 in maize.aBIOTECH · 2026Article
- Visualizing application of RUBY-MAT system in maize genetic transformation and gene expression pattern detection.Plant cell reports · 2026Article
- An optimized haploid breeding strategy employing genome-edited anthocyanin markers for visual selection in tomato.Plant communications · 2026Article
- An all-in-one visual selection system for male-sterile line production in maize and rice.Plant communications · 2026Article
- Peas in Rouge: Tyrosine Supplementation Enhances RUBY Reporter Visibility inPlants (Basel, Switzerland) · 2025Article
- Decades' progress and prospects on maize functional genomics and molecular breeding.Science China. Life sciences · 2025Review
- Monitoring the stability of transgene expression in lettuce using the RUBY reporter.Plant cell reports · 2025Article
- Walnut genome editing: an optimized CRISPR/Cas9 platform with superior genotypes and endogenous promoters.Horticulture research · 2025Article
- Precise pigment biosynthesis for flower color design inHorticulture research · 2025Article
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16 authors.
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No grant is acknowledged in the PubMed record.
Abstract
In vivo haploid induction has been extended from maize to monocotyledonous plants like rice, wheat, millet and dicotyledonous plants such as tomato, rapeseed, tobacco and cabbage. Accurate identification of haploids is a crucial step of doubled haploid technology, where a useful identification marker is very pivotal. R1-nj is an extensively used visual marker for haploid identification in maize. RFP and eGFP have been shown to be feasible in identifying haploid. However, these methods are either limited to specific species, or require specific equipment. It still lacks an efficient visual marker that is practical across different crop species. In this study, we introduced the RUBY reporter, a betalain biosynthesis system, into maize and tomato haploid inducers as a new marker for haploid identification. Results showed that expression of RUBY could result in deep betalain pigmentation in maize embryos as early as 10 days after pollination, and enabled 100% accuracy of immature haploid embryo identification. Further investigation in tomato revealed that the new marker led to deep red pigmentation in radicles and haploids can be identified easily and accurately. The results demonstrated that the RUBY reporter is a background-independent and efficient marker for haploid identification and would be promising in doubled haploid breeding across different crop species.
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