Evidence map›Paper›PMID 37195494›Full record

ArticleEuropean biophysics journal : EBJ2023

Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid.

Khalil Abu Hammad, Vlad Dinu, Thomas E MacCalman, Jacob Pattem, Margaret Goodall, Richard B Gillis, Roy Jefferis, Stephen E Harding

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in European biophysics journal : EBJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Khalil Abu HammadNational Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
Vlad DinuNational Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
Thomas E MacCalmanNational Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
Jacob PattemNational Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
Margaret GoodallInstitute of Immunology & Immunotherapy, College of Medical & Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK.
Richard B GillisNational Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
Roy JefferisInstitute of Immunology & Immunotherapy, College of Medical & Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK.
Stephen E HardingNational Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK. steve.harding@nottingham.ac.uk.ORCID http://orcid.org/0000-0002-7798-9692

Funding

Biotechnology and Biological Sciences Research Council BB/T006404/1
6 · The paper itself

Abstract

The solution properties of two different glycoforms of IgG1 (IgG1Cri and IgG1Wid) are compared using primarily sedimentation equilibrium analysis with two complementary analysis routines: SEDFIT-MSTAR and MULTISIG. IgGCri bears diantennary complex-type glycans on its Fc domain that are fully core fucosylated and partially sialylated, whilst on IgGWid, they are non-fucosylated, partially galactosylated and non-sialylated. IgGWid is also Fab glycosylated. Despite these differences, SEDFIT-MSTAR analysis shows similar weight average molar masses M

Indexed as

Immunoglobulin GPolysaccharidesGlycosylationPhysical PhenomenaImmunoglobulin GPolysaccharidesc(M)-distributionc(s)-distributionFc/Fab glycansMULTISIGSEDFIT-MSTAR

Identifiers

PMID37195494
PMCPMC10444637

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.