Evidence map›Paper›PMID 37195490›Full record

ArticleBiotechnology letters2023

Bone marrow mesenchymal stem cell-derived exosomes loaded with miR-26a through the novel immunomodulatory peptide DP7-C can promote osteogenesis.

Shuang Lai, Li Deng, Cong Liu, Xinlun Li, Liyuan Fan, Yushu Zhu, Yiling Yang, Yandong Mu

Abstract read
PubMed Publisher
In one paragraph

Article in Biotechnology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed, 1 pooled it
3.8field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 1 synthesis or guideline pooled it, 25 citations in OpenAlex.

  1. Pooled it
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  8. Review
  9. Mesenchymal stem cell-derived extracellular vesicles in periodontal bone repair.Journal of molecular medicine (Berlin, Germany) · 2025
    Review
  10. Review
  11. Article
  12. Emerging roles of exosomes in oral diseases progression.International journal of oral science · 2024
    Review
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  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Shuang LaiDepartment of Stomatology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic and Technology of China, Chengdu, 611731, Sichuan, China.
Li DengDepartment of Stomatology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic and Technology of China, Chengdu, 611731, Sichuan, China.
Cong LiuDepartment of Stomatology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic and Technology of China, Chengdu, 611731, Sichuan, China.
Xinlun LiSouthwest Medical University, Luzhou, 646000, Sichuan, China.
Liyuan FanDepartment of Stomatology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic and Technology of China, Chengdu, 611731, Sichuan, China.
Yushu ZhuSouthwest Medical University, Luzhou, 646000, Sichuan, China.
Yiling YangSouthwest Medical University, Luzhou, 646000, Sichuan, China.
Yandong MuDepartment of Stomatology, Sichuan Provincial People's Hospital, School of Medicine, University of Electronic and Technology of China, Chengdu, 611731, Sichuan, China. muyd@uestc.edu.cn.ORCID http://orcid.org/0000-0002-1829-6116
University of Electronic Science and Technology of China · CNSouthwest Medical University · CN

Funding

National Natural Science Foundation of China 82071168Sichuan Province Science and Technology Support Program 2021YFS0009
6 · The paper itself

Abstract

purposeAs small bioactive molecules, exosomes can deliver osteogenesis-related miRNAs to target cells and promote osteogenesis. This study aimed to investigate miR-26a as a therapeutic cargo to be loaded into bone marrow stromal cell exosomes through a novel immunomodulatory peptide (DP7-C).

methodsAfter transfecting BMSCs with DP7-C as a transfection agent, exosomes were extracted by ultracentrifugation from the culture supernatant of miR-26a-modified BMSCs. We then characterized and identified the engineered exosomes. The effect of the engineered exosomes on osteogenesis was then evaluated in vitro and in vivo, including transwell, wound healing, modified alizarin red staining, western blot, real-time quantitative PCR, and experimental periodontitis assays. Bioinformatics and data analyses were conducted to investigate the role of miR-26a in bone regeneration.

resultsThe DP7-C/miR-26a complex successfully transfected miR-26a into BMSCs and stimulated them to release more than 300 times the amount of exosomes overexpressing miR-26a compared with the Exo

conclusionmiR-26a can be encapsulated into exosomes through DP7-C. Exosomes loaded with miR-26a can promote osteogenesis and inhibit bone loss in experimental periodontitis and serve as the foundation for a novel treatment strategy.

Indexed as

ExosomesMesenchymal Stem CellsMicroRNAsCell DifferentiationOsteogenesisMicroRNAsDP7-CExosomesmiR-26aOsteogenesis

Identifiers

PMID37195490
OpenAlexW4376871440

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.