Evidence map›Paper›PMID 37195404›Full record

ArticleVirus genes2023

Molecular characterization of Brazilian FeLV strains in São Luis, Maranhão Brazil.

Nathálya Dos Santos Martins, Ana Paula Sousa Rodrigues, Juliana Marques Bicalho, Joanna Jéssica Albuquerque, Luana Luz Reis, Luciana Luz Alves, Renata Mondego de Oliveira, Larissa Sarmento Dos Santos, Alcina Vieira de Carvalho Neta, Rudson Almeida de Oliveira and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Virus genes, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.9field-weighted citation impact, top 29% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 6 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 3 institutions in 1 country.

Nathálya Dos Santos MartinsDepartament of Pathology, State University of Maranhão, University City Paulo VI, Cx. Postal 9, Tirirical, São Luís, MA, 65055-970, Brazil.
Ana Paula Sousa RodriguesDepartment of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais, UFMG 30 123-970, Belo Horizonte, MG, Brazil.
Juliana Marques BicalhoDepartment of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais, UFMG 30 123-970, Belo Horizonte, MG, Brazil.
Joanna Jéssica AlbuquerqueDepartament of Pathology, State University of Maranhão, University City Paulo VI, Cx. Postal 9, Tirirical, São Luís, MA, 65055-970, Brazil.
Luana Luz ReisLaboratory of Genetics and Molecular Biology, Department of Chemistry and Biology, State University of Maranhão (UEMA), Praça Duque de Caxias, s/n, Morro do Alecrim, Caxias, MA, 65604-380, Brazil.
Luciana Luz AlvesLaboratory of Genetics and Molecular Biology, Department of Chemistry and Biology, State University of Maranhão (UEMA), Praça Duque de Caxias, s/n, Morro do Alecrim, Caxias, MA, 65604-380, Brazil.
Renata Mondego de OliveiraDepartament of Pathology, State University of Maranhão, University City Paulo VI, Cx. Postal 9, Tirirical, São Luís, MA, 65055-970, Brazil.
Larissa Sarmento Dos SantosDepartament of Pathology, State University of Maranhão, University City Paulo VI, Cx. Postal 9, Tirirical, São Luís, MA, 65055-970, Brazil.
Alcina Vieira de Carvalho NetaDepartament of Pathology, State University of Maranhão, University City Paulo VI, Cx. Postal 9, Tirirical, São Luís, MA, 65055-970, Brazil.
Rudson Almeida de OliveiraDepartment of Veterinary Clinics, State University of Maranhão, University City Paulo VI, Cx. Postal 9, Tirirical, São Luís, MA, 65055-970, Brazil.
Rafael Cardoso CarvalhoCenter for Biological and Health Sciences, Federal University of Maranhão, UFMA, Rodovia BR 222, Km 04, s/n, Boa Vista, Chapadinha, MA, 65500-000, Brazil.
Ferdinan Almeida MeloDepartament of Pathology, State University of Maranhão, University City Paulo VI, Cx. Postal 9, Tirirical, São Luís, MA, 65055-970, Brazil.
Jenner Karlisson Pimenta Dos ReisDepartment of Preventive Veterinary Medicine, School of Veterinary Medicine, Federal University of Minas Gerais, UFMG 30 123-970, Belo Horizonte, MG, Brazil.
Ana Lucia Abreu-SilvaDepartament of Pathology, State University of Maranhão, University City Paulo VI, Cx. Postal 9, Tirirical, São Luís, MA, 65055-970, Brazil. abreusilva.ana@gmail.com.
Universidade Estadual do Maranhão · BRUniversidade Federal de Minas Gerais · BRUniversidade Federal do Maranhão · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The feline leukemia virus (FeLV) belongs to the Retroviridae family and Gammaretrovirus genus, and causes a variety of neoplastic and non-neoplastic diseases in domestic cats (Felis catus), such as thymic and multicentric lymphomas, myelodysplastic syndromes, acute myeloid leukemia, aplastic anemia, and immunodeficiency. The aim of the present study was to carry out the molecular characterization of FeLV-positive samples and determine the circulating viral subtype in the city of São Luís, Maranhão, Brazil, as well as identify its phylogenetic relationship and genetic diversity. The FIV Ac/FeLV Ag Test Kit (Alere™) and the commercial immunoenzymatic assay kit (Alere™) were used to detect the positive samples, which were subsequently confirmed by ELISA (ELISA - SNAP® Combo FeLV/FIV). To confirm the presence of proviral DNA, a polymerase chain reaction (PCR) was performed to amplify the target fragments of 450, 235, and 166 bp of the FeLV gag gene. For the detection of FeLV subtypes, nested PCR was performed for FeLV-A, B, and C, with amplification of 2350-, 1072-, 866-, and 1755-bp fragments for the FeLV env gene. The results obtained by nested PCR showed that the four positive samples amplified the A and B subtypes. The C subtype was not amplified. There was an AB combination but no ABC combination. Phylogenetic analysis revealed similarities (78% bootstrap) between the subtype circulating in Brazil and FeLV-AB and with the subtypes of Eastern Asia (Japan) and Southeast Asia (Malaysia), demonstrating that this subtype possesses high genetic variability and a differentiated genotype.

Indexed as

Cat DiseasesImmunodeficiency Virus, FelineAnimalsBrazilCatsGenotypeLeukemia Virus, FelinePhylogenyPolymerase Chain ReactionELISAFeline leukemia virus (FeLV)Phylogenetic diversityPolymerase chain reaction

Identifiers

PMID37195404
PMCPMC10189217
OpenAlexW4376871029

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.