Optimization of a non-activating medium for short-term chilled storage of barramundi (Lates calcarifer) testicular spermatozoa.

Adrien F Marc, Jarrod L Guppy, Hayley Marshall, Dean R Jerry, Donna Rudd, Damien B B P Paris

Open access · hybridAbstract read

In one paragraph

Article in Fish physiology and biochemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

0.8field-weighted citation impact, top 29% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 3 institutions in 2 countries.

Adrien F MarcGamete and Embryology (GAME) Laboratory, College of Public Health, Medical & Veterinary Sciences, James Cook University, Townsville, QLD, 4811, Australia. adrien.marc@my.jcu.edu.au.ORCID http://orcid.org/0000-0002-9364-6438

Jarrod L GuppyCentre for Sustainable Tropical Fisheries and Aquaculture, College of Science and Engineering, James Cook University, Townsville, QLD, 4811, Australia.ORCID http://orcid.org/0000-0003-1864-9644

Hayley MarshallCollege of Public Health, Medical, and Veterinary Sciences, James Cook University, Townsville, QLD, 4811, Australia.

Dean R JerryCentre for Sustainable Tropical Fisheries and Aquaculture, College of Science and Engineering, James Cook University, Townsville, QLD, 4811, Australia.ORCID http://orcid.org/0000-0003-3735-1798

Donna RuddCollege of Public Health, Medical, and Veterinary Sciences, James Cook University, Townsville, QLD, 4811, Australia.ORCID http://orcid.org/0000-0002-4895-1791

Damien B B P ParisGamete and Embryology (GAME) Laboratory, College of Public Health, Medical & Veterinary Sciences, James Cook University, Townsville, QLD, 4811, Australia.ORCID http://orcid.org/0000-0001-7329-8985

James Cook University · AUAustralian Research Council · AUAustralian Institute of Tropical Health and Medicine · AU

Funding

James Cook University Large Partnership Grant 2016

6 · The paper itself

Abstract

Reliable short-term chilled sperm storage is a critical prerequisite to using advanced reproductive techniques for captive breeding of barramundi (Asian sea bass; Lates calcarifer). Marine Ringer's solution (MRS) is a common non-activating medium (NAM) and has previously been used to store sperm from wild-caught barramundi. However, MRS-stored spermatozoa from captive-bred barramundi were observed to lyse within 30 min incubation. Therefore, this study aimed to optimize the composition of NAM for short-term chilled storage by characterizing and mimicking the biochemical profile of seminal and blood plasma of captive-bred barramundi. To further understand the effect of each component, osmolality was first examined to determine its effect on sperm viability. Thereafter, the effects of NaHCO

Indexed as

PerciformesSemen PreservationAnimalsHEPESMaleSemenSpermatozoaSperm MotilityHEPESBiochemicalComputer-assisted sperm analysisExtenderFishFlow cytometrySperm quality

Identifiers

PMID37193910

DOI10.1007/s10695-023-01191-8

PMCPMC10415525

OpenAlexW4376871175

What OpenQuestion holds

Textmetadata

LicenceCC BY

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.